The One

Topics: Agar plate, Staphylococcus, Staphylococcus epidermidis Pages: 9 (2752 words) Published: April 24, 2013
Fayetteville State University
Bio 430
Dr. Allen

Spray it or wipe it!!!
The scientific study on the effectiveness of CiDecon® Plus Wipes vs. CIDECON ® Q Aerosol on a mixed culture of bacteria (Serratia marcescens Staphylococcus epidermidis) living on publicly used door knobs

Fateema Thomas
Andrea Donaldson
Aryan Jaimangal
Tiffany McNeil
John Posey

Germs are a mist in every corner of the world. They are stealth and literally, are on everything. This can range from your cooking ware, or maybe you’re doorknobs, and even your fingertips. But exactly what can did mean for our human race? Are these bacterium good or bad? Bacterium sometimes can affect us in positive and negative lights. We can use this to our advantage in terms of symbioses. Some bacteria can cause disease, while some can fight them off and enhance your immune system. Staphylococcus epidermidis and Serratia marcescens are both bacterium that are known to do good, but also bad for the body. This is why aseptic technique is so important in our everyday life. But, which is the best method? In current decade, sprays and wipes, have lead the way in the innovation of disinfectants. So our group decided to base our expirement on this sole idea, which sterilization is more accurate, disinfectant wipes or spray? We cultured a mix of S. epidermidis and S. marcescens as our control. For the most part, the control flourished and we were ready to start testing. We hypothesized the spray would disinfect with a greater percentage than the wipes. We conducted four trails of placing the bacterium mix on 3 styles of door handles. Since the doors knobs had two sides, we would place the same control on both sides of the knob and disinfect each side with different disinfectants. There were intervals of 4 minutes, in which we let the bacteria sit, and swabbed the bacteria off. Immediately after, we inserted the sample on a nutrient agar dish. Finally, we incubated the samples for approximately 2 days. To our prevail, we noticed that both disinfectants were approximately the same. Both simultaneously disinfected the door knobs almost perfectly. Our samples thrived, yet there was a foreign bacterium in the plates. I guess that is why these disinfectants are only 99.999% effective. Over all the experiment was excellent. The only error that might have been made could have been not doing another trail. (ERRORS)

Table of Contents

AbstractPage 2
Table of ContentsPage 3

AcknowledgementsPage 4

Research PaperPage 5-17

Reference Page 18

Dr. Allen,
Thank you for the option of creating our own lab and supplying the tools to make it happen.

In an everyday world, contact is the greatest action that takes place between two or more objects. As humans, we make contact with every and anything, but do we really think about what we are touching? When you really ponder the thought, millions of bacteria grow on the littlest of areas, for example a door knob. Door knobs are continuously engaged with contact approximately every 10 seconds. (1) People carry all kinds of products such as, foreign bacteria, food, and even open wounds on their hands when touching door knobs. Any one of these can leave a footprint upon the knob and start the process in bacterium growth.

There and plenty of bacterium that have been proven to live on the body such as, Staphylococcus. Staphylococcus aureus and Staphylococcus epidermidis are significant in their interactions with humans. S. aureus colonizes mainly in moist, hot places in the body, but upon contact, this bacterium is likely to thrive on any surface. Thus, it can carry out to numerous surfaces, such as a door knob, and transfer to anybody. S epidermidis is highly adapted to the diverse environments of a human host. S epidermidis is almost found approximately 90% on...
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