The Effect of Temperature on The Activity of The Enzyme Catalase
Introductions: Enzmyes are proteins that catalyze chemical reactions. All living things have catalase present in them. Catalase is a common HYPERLINK "http://en.wikipedia.org/wiki/Enzyme" enzyme found in nearly all living organisms that are exposed to oxygen, where it HYPERLINK "http://en.wikipedia.org/wiki/Catalyst" catalyzes the decomposition of HYPERLINK "http://en.wikipedia.org/wiki/Hydrogen_peroxide" hydrogen peroxide, which is damaging to the cells, into water and HYPERLINK "http://en.wikipedia.org/wiki/Oxygen" oxygen. My investigation is the effect of temperature on the activity of enzyme catalase. In this investigation I wanted to determine the effect that temperature has on the enzyme catalase.
Question: What is the effect of temperature on catalase enzyme activity?
Dependant- Amount of gas produced
-Amount of liquid left in test tube
Controlled -Amount of Hydrogen Peroxide
-Amount of potato enzyme
-Hot temperature water bath (set at 90 degrees C)
-Cold temperature water bath (set at .5 degrees C)
-Room temperature water bath (set at 15 degrees C)
- Hydrogen Peroxide
-5 small test tubes (5.2 ml each)
-5 regular size test tubes
Collect all of materials on the materials list
Make Potato Enzyme. To make this take 100 grams of potato and add 200 grams of water. Blend in a blender till liquified. Then filter liquid through 2 layers of cheese cloth. Once complete keep in the refridgerator at 40 degrees.
Set up all water baths to correct temperatures. Set hot water bath at 90 degrees C, Cold water bath at .5 degrees C, and room temperature water bath at 15 degrees C
Set up 5 small clean 5.2 ml test tubes on... [continues]
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