The Effect of Temperature on the Activity of the Enzyme Catalase

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Science IA Rough Draft

The Effect of Temperature on The Activity of The Enzyme Catalase

Introductions: Enzmyes are proteins that catalyze chemical reactions. All living things have catalase present in them. Catalase is a common  HYPERLINK "http://en.wikipedia.org/wiki/Enzyme" enzyme found in nearly all living organisms that are exposed to oxygen, where it  HYPERLINK "http://en.wikipedia.org/wiki/Catalyst" catalyzes the decomposition of  HYPERLINK "http://en.wikipedia.org/wiki/Hydrogen_peroxide" hydrogen peroxide, which is damaging to the cells, into water and  HYPERLINK "http://en.wikipedia.org/wiki/Oxygen" oxygen. My investigation is the effect of temperature on the activity of enzyme catalase. In this investigation I wanted to determine the effect that temperature has on the enzyme catalase.

Question: What is the effect of temperature on catalase enzyme activity?

Variables-
        Independent- Temperature

        Dependant- Amount of gas produced
       -Amount of liquid left in test tube
        Controlled -Amount of Hydrogen Peroxide
                      -Amount of potato enzyme

Materials
-Hot temperature water bath (set at 90 degrees C)
-Cold temperature water bath (set at .5  degrees C)
-Room temperature water bath (set at 15 degrees C)
- Hydrogen Peroxide
- Potato
-Blender
-Cheese cloth
- Water
-Pipette
-5 small test tubes (5.2 ml each)
-5 regular size test tubes
-Paper
-Pencil
-Refrigerator
-Thermometer

Method
Collect all of materials on the materials list
Make Potato Enzyme. To make this take 100 grams of potato and add 200 grams of water. Blend in a blender till liquified. Then filter liquid through 2 layers of cheese cloth. Once complete keep in the refridgerator at 40 degrees. Set up all water baths to correct temperatures.  Set hot water bath at 90 degrees C, Cold water bath at .5 degrees C, and room temperature water bath at 15 degrees C Set up 5 small clean 5.2 ml test tubes on rack. Add to each test tube 2 ml hydrogen peroxide and 2 ml of the chilled potato enzyme. Invert the filled 5.2 ml test tubes into larger test tubes

Place the 5 inverted test tubes into the room temperature bath Wait 15 minutes, make sure during all time water stays at correct temperature Take the all the inverted test tubes out of the water bath.

Take test tues over to the sink and extract the small 5.2 ml test tube from the larger test tube. Measure the remaining liquid in each of the 5 small test tubes and record the amount in mL Subtract the amount of the remaining liquid in the test tubes from the 5.2 mL initial volume to get the amount of gas produced and record in table. Wash out all test tubes and repeat steps 4-11 for cold water bath Wash out all test tubes and repeat steps 4-11 for hot water bath Clean out all supplies and place back in correct spots

Data Table

Processed Data

Overview-
I performed calculations on the data to test if the null hypothesis was valid. The null hypothesis in this case is that temperature does not have an impact on the rate on enzyme activity. It turned out that this was not the case and temperature had a significant effect on enzyme activity. To determine this I calculated the mean, the standard deviation, the T test, the degrees of freedom, and then compared the T value to the critical value at the 95% confidence limit.

Sample Calculations-

        Mean (Average)

EMBED Equation.3 = EMBED Equation.3

EMBED Equation.3 = mean (average)
xi = individual values in data set
( = sum of values
N = number of values in data set

Standard Deviation
s = EMBED Equation.3

s = standard deviation
xi = individual values in the data set
EMBED Equation.3 = mean of all values in the data set
N-1 = number of values in data set minus 1 (=Degrees of Freedom)

Calculations

Room Temperature

Mean- EMBED Equation.3 = 5.2+4.7+3.7+3.8+5.2

5

EMBED Equation.3 =4.52...
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