The Effect of Temperature on Renin

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Background information:
Catalysts are substances that speed up the chemical reaction by decreasing the activation energy. In biology catalyst is called biological catalyst, and enzyme is an example of biological catalyst. Enzyme is very important for biochemical reactions, as it helps the reaction to become faster than usual. The reactions with enzymes will react 10 billion times faster than those solutions without enzymes. (clitoncc)The enzymes can only react with one particular reaction, in order for the reaction to react it needed to find the correct enzyme. (uab) Rennin is an enzyme produced in the inner lining of the abomasum. Renin will solidify milk and makes it curdle. Curdling of milk is essential for the proper digestion of milk proteins in the stomach. (buzzle)

Aim:
To analyze the effect of temperature on Renin and to find the best temperature for enzyme activity Hypothesis:
Renin works the best in which the rate of reaction is at the highest or optimum, in 40˚C Materials:
Bunsen burner
Wire gauze
Thermometer
Dropper
Mortar
Pestle
Crushed ice
Distilled water
Test-tubes
Measuring cylinder
Beaker
Milk
Junket tablet
Test tube holder
Stopwatch

Procedures:
1.Junket tablet are crushed with the help of mortar and pestle in addition with 10 ml of water. 2.The temperature of water bath is checked accordingly.
3.Other material is prepared, such as: test tubes, milk, thermometers, measuring cylinders and Bunsen burner. 4.3ml of milk is poured in each test tube (4).
5.Test tubes then are inserted into the water bath. As the milk suited the temperature desired, 1 ml of renin is added to the first 3 test tubes, while the last one is used as a negative control. 6. All of the tubes is examined and record for every minute for 20 minutes. 7.Steps are repeated for 2 times.

Table Results

TemperatureTube 1 (trial 1)Tube 2
(trial 2)Tube 1
(trial 1)Tube 2
(trial 2)Average timeRate of...
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