The Catalase Enzyme

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The purpose of the lab is to observe the breakdown of hydrogen peroxide by the liver enzyme catalase. Determine the factors, such as increased concentration, varying temperature, and changes in state, influence how quickly the reaction takes place, and how well the enzymes function. Hypothesis:

For Varying Enzyme concentration, for groups A,B,C, and D, if there is an increase in enzyme concentration as a result of the increasing varied number of discs, then there will be an increased rate of reaction as a result of more active sites being available. For Varying Temperature, for groups E and F, if there is an increase in temperature, then there is an increase in the enzyme activity up to a critical point, and vice versa. For Varying State of Enzyme, if the enzyme is boiled, then the protein is denatured, destroying the active sites, therefore no reaction occurs with the hydrogen peroxide. Observations:

Varying Enzyme Concentration
GroupControlVaried Number of Discs
A140 mL/minute4158 mL/minute
B187 mL/minute6388 mL/minute
C130.5 mL/minute8365.8 mL/minute
D157.1 mL/minute10381.05 mL/minute
Varying Temperature
E165 mL/minute0oC103 mL/minute
F86.67 mL/minute40oC140.67 mL/minute

Varying State of Enzyme
GroupControlTreating Enzyme
G153 mL/minuteBoiled0


Enzyme Concentration
Increase in Temperature
Decrease in Temperature
Treated Enzyme (Boiled)


A)Enzyme Concentration – It is known that if there is an increase in enzyme concentration, then there will be an increased rate of reaction as a result of more active sites being available. Since we increased the number of discs to increase the concentration, we provided more surface area for the enzyme catalase to break down the hydrogen peroxide. From the results obtained from Group A,B,C and D, shown through the graphs, one can see that as...
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