The Affects of Temperature on Amount of Oxygen, Mung Beans (Vigna Radiata) Consume During Cellular Respiration

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Amount of Oxygen, Mung Beans (Vigna Radiata) Consume during Cellular Respiration

Introduction:

Cellular respiration is a catabolic reaction that refers to the process of converting chemical energy of organic molecules into a simplify form so it can be used immediately by organism. Glucose may be oxidized completely if sufficient oxygen is available, by the following equation: C6H12O6 + 36 ADP + 36Pi + 6O2(g) 6 H2O + 6 CO2(g) + 38 ATP + heat All organisms, including plants and animals, oxidize glucose for energy. Often, this energy is used to convert ADP and phosphate into ATP. The process of complete oxidation involves glycolysis, Krebs cycle and electron transport chain. Besides ATP, pyruvate molecules, NADH and FADH2 will be generated to aid the process in aerobic respiration. These chains of reactions happen in cytoplasm. It enters into mitochondrial matrix and releases its final product in the form of energy, water and carbon dioxide. In balancing the process, oxygen is being consumed (Campbell, 2008). In this experiment, Mung bean seeds (Vigna Radiata) were used to measure the rate of cellular respiration. The consumption of oxygen by Mung bean seeds will be measured as weight specific respiration by measuring volume of oxygen consumed, incubation time and weight of Mung bean seeds (ml h-1 kg-1). A respirator will be used to regulate the external and internal air pressure. Carbon dioxide will be produced as oxygen is consumed therefore to eliminate pressure from effecting the measurement, a chemical will be added that will selectively remove CO2. In order to regulate the external pressure that might force changes in the respirator, a closed system was implemented. Potassium hydroxide, KOH, will chemically react with CO2 by the following equation: (Frankel, 2005) 2 KOH + CO2 K2CO3H2O

There were a few objectives in this experiment. The first objective is to determine whether the germinating Mung bean seeds go through cellular respiration. Second objective is to find out if different temperature, affect respiration rate and what are the reasons for the differences. It is hypothesize that, the Mung bean seeds incubated at 200C temperature will require more oxygen consumption due to more activity mediated by enzymes. The weight specific respiration of the Mung bean seeds will be greater in the 200 C treatment. By the end of this experiment, we would be able to see the influences of temperature on cellular respiration. Understands how oxygen demand in relationship to the amount of organic materials available and finally, how KOH absorbs CO2.

Methods:

To study the effects of temperature, we measured the amount of oxygen being consumed by Mung bean seeds and observed the differences with 100 C and 200 C water bath. There were certain steps that were followed in order to conduct this experiment. In preparation for this experiment, a respirometer was the primary device used. Two respirometers were prepared for two treatments. In one respirometer, two test tubes were needed. One as the primary experiment and one served as a control. This piece of equipment, consist of a tube with a rubber stopper to seal off the top. The rubber stopper was equipped with a 1ml pipet and a syringe that helped regulate air pressure without allowing air into or out of the tube, as in a closed system (Danyk, 2012). Before the experiment could begin, several steps were taken. In the respirometer, the pipet space was sealed with a drop of 10% KOH, using a dropper. The syringe was used to manipulate and regulate the KOH so that the bottom of the drop, should sit at zero. Mung bean seeds were added to one test tube and left off 3cm gap from the top rim. Another test tube was filled with absorbent cotton. A few drops of water were dropped to moisten the cotton. Both test tubes were closed with the stopper. Both tubes were incubated into the water bath of 100 C. The same preparation was repeated with a 200 C treatment....
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