Task 11
When comparing the sample to the wet mount slides, they look very similar. However in the staining methods, the cells and their borders were very distinct while the one in the wet mounts were not as distinct. It was a bit challenging to determine the figures in the cheek smear, but the bacteria form in the yeast wet mount were easily recognized.
The direct staining was the following technique used. The cells were very easy to assess, well demarcated and had a very distinctive color. Instead of the background and around the cell in the cheek smear, the indirect staining way seemed to in fact tint the cells.
Identification of the yeast cells by the indirect system was done effortlessly while the cells in the plaque were not that easy to recognize. After comparison, the deduction is that the direct staining offers the most distinct view of the cells.
With the use of certain mount methods, the shape of the bacteria were easier to determine is some slides. Cocci could be seen in the wet mount of yeast, but in the wet mount cheek smears it was not that easy to see the morphology of the cells. The plaque smear have cocci and bacillus. On the direct stain slides the cheek smear and the yeast slide have cocci. Also on the yeast slide with indirect stain cocci were observed.
The quality of the dye will determine the outcome for the difference in direct and indirect stains. Indirect staining uses dyes such as Congo red, nigrosin or India ink. The yeast smears look the same with every staining technique but, they were more visibly demarcated and more evident when directly stained. Using indirect staining, the cells in the cheek smear slide looked as if they were stained, while in the direct staining slide the cells are sharper. Looking at the plaque slide, in the direct staining the bacteria look like having spiral formed but in the indirect staining slide it seems that the bacteria are rod-shaped.
The yeast smears have cocci, typically in groups and
The direct staining was the following technique used. The cells were very easy to assess, well demarcated and had a very distinctive color. Instead of the background and around the cell in the cheek smear, the indirect staining way seemed to in fact tint the cells.
Identification of the yeast cells by the indirect system was done effortlessly while the cells in the plaque were not that easy to recognize. After comparison, the deduction is that the direct staining offers the most distinct view of the cells.
With the use of certain mount methods, the shape of the bacteria were easier to determine is some slides. Cocci could be seen in the wet mount of yeast, but in the wet mount cheek smears it was not that easy to see the morphology of the cells. The plaque smear have cocci and bacillus. On the direct stain slides the cheek smear and the yeast slide have cocci. Also on the yeast slide with indirect stain cocci were observed.
The quality of the dye will determine the outcome for the difference in direct and indirect stains. Indirect staining uses dyes such as Congo red, nigrosin or India ink. The yeast smears look the same with every staining technique but, they were more visibly demarcated and more evident when directly stained. Using indirect staining, the cells in the cheek smear slide looked as if they were stained, while in the direct staining slide the cells are sharper. Looking at the plaque slide, in the direct staining the bacteria look like having spiral formed but in the indirect staining slide it seems that the bacteria are rod-shaped.
The yeast smears have cocci, typically in groups and
References: Betsy, Tom Keogh, James. (2005). Microbiology Demystified. Blacklick, OH, USA: McGraw-Hill Professional Publishing. WWW. Labpad.com. Englewood, CO US: Hands on Lab Inc.