Synthesis and IR Analysis of trans-[Bis(inosinato)palladium(II)]
In this lab we synthesized trans-[Bis(inosinato)
palladium(II)] for IR analysis. After completion of the reaction 84.4% of the product was collected. IR spectrum of the product and inosine was analyzed to determine how the inosine is bonded to the metal, palladium. From the IR analysis, it was determined that when inosine reacts with palladium, a shift of about 65 cm-1 in the carbonyl stretch was observed.
In this experiment, trans-[Bis(inosinato)palladium(II)], was synthesized. The general reaction equation for the reaction is shown below: KOH
(1) K2[PdCl4] + 2 inosine trans-[Pd(inosine-H+)2] + 2KCl + 2HCl
The IR spectrum of the product and inosine was analyzed to compare the location of the carbonyl bond when inosine interacts with a metal such as palladium. This study is important because the interaction of metals with an organic base purine derivative, such as inosine has great biological significance.1 For instance, many cofactors in various enzymatic reactions are both metal ions and nucleosides, such as organic base purine derivatives.1 By studying the infrared data for the boinding of insoine to the metal atom, the interactions between the purine derivative and metal can be studied.
All reagents and solvents used were purchased from commercial suppliers. All solvents and reagents used in this experiment were used as received. To obtain IR spectra, Thermo Scientific Nicolet IR100 FT-IR spectrometer was used.
General Procedure 1: Preparation of trans-[Bis(inosato)palladium(II)]¹
Inosine (0.17 mmol) was placed in a beaker containing 5 mL of water and a magnetic stirring bar. Another solution was prepared by dissolving potassium tetrachloropalladate(II) (0.1 mmol) in 1 mL of distilled water. This solution was added slowly dropwise with a pipet to the beaker.
The two solutions were...
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