Student Doctor
HEMIN TEST
Introduction Ludwig Karl Teichmann was a Polish anatomist and physician who made an enduring contribution to forensic science with his discovery of the Hemin test or Teichmann test for hemoglobin. Also called the Teichmann crystal, this is a test that is used on dried stains to determine whether or not blood is present. Dr. Teichmann made his forensic discovery in 1853. His microcrystalline test remains in use today as a means of identifying whether or not dried stains at a crime scene, on clothing or other fabric, or elsewhere at the site of a forensic investigation contain human blood.
Objectives 1. Discuss the biochemical basis of blood detection using Hemin Test 2. Determine the clinical significance blood properties and the components identified
Materials 1. Glass Slide 2. Needle and Lancet 3. Glacial Acetic Acid 4. 0.1 NaCl 5. Bunsen Burner 6. Microscope
Procedure 1. Defibrinated blood was smeared on a glass slide to form a thin film. 2. The glass slide was passed over a moderate flame back and forth until the blood was quite dry. 3. Two drops of glacial acetic acid containing 0.1 N sodium chloride was then added to the film. 4. After placing a cover slip over the film of blood, the glass slide was then heated over a low flame until bubbles appeared and boiling took place. 5. The glass slide was then removed from the flame and another drop of 0.1 N sodium chloride was added to the film. 6. The slide was allowed to cool and film was observed under a microscope.
Results
(Hi Jess, please insert the photo na lang.)
Figure 1. Formation of rhomboid crystals or hemin
Upon observation on the microscope, dark rhomboid crystals or hemin was formed. This confirmed the presence of blood.
Discussion Hemin Test is a confirmatory test in identifying blood stain samples which is of
Introduction Ludwig Karl Teichmann was a Polish anatomist and physician who made an enduring contribution to forensic science with his discovery of the Hemin test or Teichmann test for hemoglobin. Also called the Teichmann crystal, this is a test that is used on dried stains to determine whether or not blood is present. Dr. Teichmann made his forensic discovery in 1853. His microcrystalline test remains in use today as a means of identifying whether or not dried stains at a crime scene, on clothing or other fabric, or elsewhere at the site of a forensic investigation contain human blood.
Objectives 1. Discuss the biochemical basis of blood detection using Hemin Test 2. Determine the clinical significance blood properties and the components identified
Materials 1. Glass Slide 2. Needle and Lancet 3. Glacial Acetic Acid 4. 0.1 NaCl 5. Bunsen Burner 6. Microscope
Procedure 1. Defibrinated blood was smeared on a glass slide to form a thin film. 2. The glass slide was passed over a moderate flame back and forth until the blood was quite dry. 3. Two drops of glacial acetic acid containing 0.1 N sodium chloride was then added to the film. 4. After placing a cover slip over the film of blood, the glass slide was then heated over a low flame until bubbles appeared and boiling took place. 5. The glass slide was then removed from the flame and another drop of 0.1 N sodium chloride was added to the film. 6. The slide was allowed to cool and film was observed under a microscope.
Results
(Hi Jess, please insert the photo na lang.)
Figure 1. Formation of rhomboid crystals or hemin
Upon observation on the microscope, dark rhomboid crystals or hemin was formed. This confirmed the presence of blood.
Discussion Hemin Test is a confirmatory test in identifying blood stain samples which is of
References: Citation: From books: Murray, R. (2003). Harper 's illustrated biochemistry. (26th ed.). McGhaw-Hill. Nelson, D., & Cox, M. (2008). Lehninger principles of biochemistry. (5th ed.). New York: W.H. Freeman and Company. From online: http://www.wpahs.org/specialties/bloodless-medicine/what-is-hemin-how-is-it-used http://www.enotes.com/topics/teichmann-ludwig-karl/reference