Stress and Cellular Membranes

Stress and Cellular Membranes
Bio Lab
SBI 4U1

Due: Sept 24th, 2010
For: Ms. Barton
By: Ameema Saeed
Introduction:
(See attached lab)

Purpose:
(See attached lab)

Hypotheses: Tap water will cause no damage to the membranes. The extreme temperatures of the water cooled to 0°C, and heated to 94-100°C will greatly damage the membranes. Extreme heat will be more damaging than extreme cold. The more concentrated the solvent, the more damage it will cause to the membranes, therefore the solvent of 50% Acetone, and the solvent of 50% Methanol will have higher readings of damage than the solvents of 25% Acetone, and 25% Methanol.

Materials:
(See attached lab)

Procedure:
(See attached lab)

Observations:

Temperature Lab
| |Quantitative results |Qualitative results |
|Room temperature tap water |0.045 |Water is exactly the same. |
|60˚ C water |0.313 |Water is dark Pink. |
|Boiling water (94˚ C-100˚ C) |0.520 |Water is darkest Pink, Beet is soft and mushy. |
|Cold water (0˚ C) |0.267 |Water is dark Pink, close in colour to the 60˚ Beet. Beet looks |
| | |less damaged. |

Solvent-related Treatment lab
| |Quantitative results |Qualitative results |
|Regular tap water |0.038 |Water is exactly the same. |
|25% Acetone |0.061 |Water is slightly changed in colour—light Pink.

Bibliography: - Andrews, C. J. (1996). How do plants survive ice? Annals of Botany, 78. Retrieved from http://aob.oxfordjournals.org/content/78/5/529.full.pdf -Class notes -Hunt, I. Lipids. University of Calgary- Chemistry. Retrieved September 23, 2010, from http://www.chem.ucalgary.ca/courses/351/Carey5th/Ch26/ch26-0.html -Yang, W. H., Cenkowski, S., & Wang, H.S. (1996). Microwave-enhanced denaturation of sugar beet tissues. American Society of Agricultural and Biological Engineers Technical Library. Retrieved from http://asae.frymulti.com/abstract.asp?aid=27587&t=1