Stick Cane Phytochemical Analysis and Antibacterial Test

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CHAPTER I:
INTRODUCTION
Background of the study
Stickcane also known as Carizo, Arundo, Spanish cane or giant reed, is widely known in the Philippines. It is usually used as trellis. It is a strong candidate for use as renewable biofuel source because of its fast growth rate, ability to grow in different soil types and climatic conditions. This can provide a superior renewable energy biomass source of environmental benefits. It provides below ground carbon sequestion in addition to its above ground up take due to its rapid growth. Arundo stems produced during the first growing season are unbranched and photosynthetic. It grows spontaneously and abundantly from heavy clays to lose sands and gravelly soils, but prefers wet drained soils where it produces monotypic dense stands, very competitive with weeds. In the Philippines, old folks use the stickcane shoots to cure newly castrated pig’s wound. This is placed inside the castrated part right after castration and is also used as medicine for ulcer. Arundo is abundant in the researcher’s place, Bolog, Kiangan, Ifugao and is used commonly as trellis for vine plants. It is for this reason that the study was conceptualized. The researcher aims to determine the antibacterial potential of stickcane (arundo donax) shoot extract and further to its phytochemical analysis.

Statement of the Problem
The researcher aims to determine the antibacterial potential of stickcane (arundo donax) shoot extract and further to its phytochemical analysis. Specifically, it answers the following:
1. What are the phytochemical content of the stickcane shoot extract? 2. Is there a significant difference in the antibacterial properties of the plant extract on Pseudomonas and Escherichia Coli as shown by the zone of inhibition? 3. Does the stickcane shoots extract exhibit antibacterial property? Significance of the Study

The result of this study can be beneficial to the community. It can motivate the mass production of stickcane in the community as a source of income. It can also give information on the other uses of stickcane. It will be also important to future researchers. As this can be a basis for future research studies. It can also lead to formulation of typical medicines for wound and others. Scope and Delimitation

The study focused on the shoot of the stickcane. It was conducted at the Ifugao Provincial Science High School Mabatobato, Lamut, and Ifugao. The antibacterial analysis and phytochemical testing was conducted at the Nueva Vizcaya State University Laboratory Bayombong, Nueva, Vizcaya.

Related Literature
I. Stickcane(Arundo Donax)
Arundo donax, Giant Cane, is a tall perennial cane growing in damp soils, either fresh or moderately saline. Other common names include Carrizo, Arundo, Spanish cane, Wild cane, and Giant reed.

It is native to Eastern and Southern Asia, and probably also parts of Africa and Southern Arabic Peninsula. It has been widely planted and naturalized in the mild temperate, subtropical and tropical regions of both hemispheres (Herrera & Dudley 2003), especially in the Mediterranean, California, the western Pacific and the Caribbean. It forms dense stands on disturbed sites, sand dunes, in wetlands and riparian habitats. It generally grows to 6 meters (20 ft), in ideal conditions it can exceed 10 metres (33 ft), with hollow stems 2 to 3 centimetres (0.79 to 1.2 in) diameter. The leaves are alternate, 30 to 60 centimetres (12 to 24 in) long and 2 to 6 centimetres (0.79 to 2.4 in) wide with a tapered tip, grey-green, and have a hairy tuft at the base. Overall, it resembles an outsize common reed (Phragmites australis) or a bamboo (Subfamily Bambusoideae) Its flowers in late summer, bearing upright, feathery plumes 40 to 60 centimetres (16 to 24 in) long, but the seeds are rarely fertile. Instead, it mostly reproduces vegetative, by underground rhizomes. The rhizomes are tough and fibrous and form knotty, spreading mats...
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