Starch Lab

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Krupa Desai
Cell Biology
April 3, 2013
Lab: Biosynthesis of Starch

Introduction: In this lab we learned the concept and procedure of synthesizing starch. We also learned the effects of pH and temperature on the reaction rates of amylase.. In the process of the synthesis lab we learned phosphorylation using a potato, which was what we synthesized. The phosphorylation took place after the addition of primer. There are two different types of starches used are amylose and amylopectin. To test for the presence of starch, the Starch Test is used. This is a simple test in which iodine is added to a given solution. If a polysaccharide such as starch is present then the iodine ion will lodge itself in the polysaccharide chain and give it a black-blue color. If iodine added to a solution turns black-blue than starch is present. If the solution remains the color of iodine, reddish-orange, there is no starch present, a negative test. In our experiment we scale the color we see in comparison to the neutrals to test for the amount of starch. Methods/Materials: See print out Data Table:Below

Time in minutes| Tube 1| Tube 2| Tube 3| Tube 4| Tube 5| Tube 6| Tube 7| 0 mins(TO)| 0| 0| 0| 0| 0| ++| +++|
3 mins| 0| +| 0| 0| +| +| +|
6 mins| +| ++| +| +| +++| +++| +++|
9 mins| 0| +++| +| 0| +++| +++| ++++|
12 mins| 0| +++| +| 0| +++| +++| ++++|
15 mins| 0| +++| +| 0| ++++| ++++| ++++|
18 mins| 0| +++| ++| 0| ++++| +++| ++++|
21 mins| 0| +++| ++| 0| ++++| +++| +++|
24 mins| 0| +++| ++| 0| +++| +++| ++++|
27 mins| 0| ++++| ++| 0| +++| +++| ++++|
30 mins| 0| ++++| ++| 0| ++++| ++| ++++|

Discussion:
This lab gives a better understanding of biosynthesis of starch. As you can see with our data we had some discrepancies in tubes where glucose was present no starch should have been found however we have “+” in a few of the time slots this could be do to...
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