Simulated Breeding Experiments with Drosophila Mendelian Genetics in Pea Plants

Topics: Allele, Gregor Mendel, Classical genetics Pages: 7 (1691 words) Published: May 2, 2013
Internal Assessment | |
A. SIMULATED BREEDING EXPERIMENTS WITH DROSOPHILA B. PROBABILITY AND MENDELIAN GENETICS IN PEA PLANTS
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Contents
Part A
Raw Data…………………………………………………………………………………………………………………...........................1 Processed Data……………………………………………………………………………………………………………………………………2 Conclusion…………………………………………………………………………………………………………………………………………..3 Part B

Raw Data……………………………………………………………………………………………………………………………………………..4 Processed Data…………………………………………………………………………………………………………………………………….5 Conclusion……………………………………………………………………………………………………………………………………………7

Part A
Raw Data
The different possible crosses in characteristics with Drosophila in eye colour and wing shape.

Table 1: Eye Colour Alleles
Parents | Eye Colour| F1| F2| | |
Wild x Wild| Wild | 1235| 1160| | |
Brown x Wild| Brown| 0| 306| | |
| Wild | 1190| 892| | |
Purple x Wild| Purple| 0| 325| | |
| Wild | 1208| 897| | |
Sepia x Wild| Sepia| 0| 285| | |
| Wild| 1204| 864| | |
| | | | | |
Parents| Eye Colour| F1| F2|
 |  | Male| Female| Male| Female |
White x Wild| White | 574| 0| 273| 296|
| Wild| 0| 618| 304| 300|

Table 2: Wing Shape alleles
Parents| Wing Shape| F1| F2|
Wild x Wild| Wild| 1741| 1490|
Curly x Wild| Curly| 645| 600|
| Wild| 665| 586|
Curved x Wild| Curved| 0| 315|
| Wild| 1206| 931|
Dumpy x Wild| Dumpy|  0| 296|
| Wild| 1182| 904|
Scalloped x Wild | Scalloped| 624| 594|
| Wild| 620| 581|

Processed Data

Chi Square| |

Table 3: Eye colour alleles Processed
Parents | Eye Colour| F1 Observed| Expected| F2 Observed| Expected| Chi Square| Degree of Freedom| P value| Wild x Wild| Wild | 1235| 1235| 1160| 1160| 0| 1| 1.00| Brown x Wild| Brown| 0| 0| 306| 299.5| 0.14| 1| 0.70| | Wild | 1190| 1190| 892| 898.5| 0.05| 1| 0.82|

Purple x Wild| Purple| 0| 0| 325| 305.5| 1.24| 1| 0.26| | Wild | 1208| 1208| 897| 916.5| 0.4| 1| 0.53|
Sepia x Wild| Sepia| 0| 0| 285| 287.2| 0.02| 1| 0.89| | Wild| 1204| 1204| 864| 861.8| 0.01| 1| 0.92|

Parents| Eye Colour| F1| F2| Expected| Chi Square| P Value|  |  | Male| Female| Male| Female | Male| Female| Male| Female| Male| Female| White x Wild| White | 574| 0| 273| 296| 288.5| 298| 0.8| 0.01| 0.36| 0.92| | Wild| 0| 618| 304| 300| 288.5| 298| 0.83| 0.01| 0.36| 0.92|

Table 4: Wing shaped alleles Processed
Parents| Wing Shape| F1| F2| Expected| Chi Square | Degree of Freedom| P value| Wild x Wild| Wild| 1741| 1490| 1490| 0| 1| 1|
Curly x Wild| Curly| 645| 600| 593| 0.08| 1| 0.77|
| Wild| 665| 586| 593| 0.08| 1| 0.77|
Curved x Wild| Curved| 0| 315| 312| 0.02| 1| 0.89|
| Wild| 1206| 931| 934| 0.01| 1| 0.92|
Dumpy x Wild| Dumpy| 0| 296| 300| 0.53| 1| 0.46|
| Wild| 1182| 904| 900| 0.18| 1| 0.67|
Scalloped x Wild | Scalloped| 624| 594| 587.5| 0.07| 1| 0.79| | Wild| 620| 581| 587.5| 0.07| 1| 0.79|

Conclusion
The results indicate, in terms of eye colour in the drosophila, that wild type dominates the other traits for the most part. In table 1, whether the wild type drosophila was crossed with brown, purple or sepia, 100% of the F1 generation was wild type. In the F2 generation, the results showed a 3:1 ratio between the wild type and either brown, purple or sepia eyes with wild type as the majority. Considering this, it can be assumed that wild type is the dominant trait in these alleles. This result is in alignment with Mendel’s Law of Segregation as it is the expected ratio when heterozygous (the children of the F1 generation) reproduce in a monohybrid cross; the dominant trait is present three times as often as the recessive trait. An exception of this...
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