EXTRACTION OF INTRACELLULARLY SYNTHESISED SILVER NANOPARTICLES USING FUNGI VERTICILLIUM TERRESTRE.
Author: Mr. Imran Latif Patel,
Co-Author: Prof. Mrs. Ragini Deshpande.
New Arts, Com. And Science college, Ahmednagar.
(University of Pune.)
Nanobiotechnolgy has become the subject of discussion at global level. Nanoparticles are proving themselves “VITAL” in various fields like biomedical sciences, magnetics, optoelectronics, nanochips production,etc. Nanoparticles are been commonly used in various drug therapies.Uptil now, pioneers from this field have atttained success of synthesising nanoparticles from micro-organisms like fungi, bacteria, yeast, etc. Nanowires and nanotubes can be prepared by extraction of this nanoparticles from their source. This study is concerned with extraction of intracellularly produced silver nanoparticles from the fungus Verticillium terrestre . Further innovations in this nanoparticles synthesis are also included in this project..
The study of nanomaterials(1nm=10-9m) has increased a lot due to their fundamental properties, organizations to appear as superstructures. Nanoparticles are having many applications such as optoelectronics2,3,catalysis4,5, reprography6, single electron transistors and light emitters 7,8, non linear optical devices9,10, photoelectrochemical applications11. They are also applied in biomedical sciences, magnetics, mechanics and energy sciences12.
Innovations in this nanoparticles will play an important role in various fields for upcoming century12. Various techniques involved in production of nanoparticles resemble intake of high capital as well as inefficient use of energy and material. Hence many researchers have turned their attention for biological systems for preparation of these nanoparticles.
Various types of organisms like bacteria, fungi, yeast, etc.are involved for intra or extra cellular biosynthesis of nanoparticles.E.g unicellular organisms like magnetotic bacteria produces magnetite nanoparticles13, diatoms synthesize siliceous materials 14. The above micro organisms are mainly used in bioleaching process. Multicellular organisms like geranium(plant) are also utilized for the synthesis of nanoparticles. Even though these micro organisms involved in toxic matter remediation , they are the best employees in nanofactories15. Thus procedures for biological production of nanoparticles have welcomed all over the world.
Organisms shows presence of hydrogenasae enzyme which reduces ions from the solution. Various fungi like verticillium 1, F.oxysporum16, actinomycete like thermomonospora1 are been used for the production of gold, silver1, iron16, nanoparticles. Fungus verticillium produces silver and gold nanoparticles when exposed to their respective salt solution. Verticillium grows at 25 0C1. Nanoparticles which are synthesized intracellularly can be observed using various methods like Scanning Electron Microscope. UV-vis spectroscopy and X-ray diffraction method. Fungus verticillium when exposed to silver nitrate solution produces intracellular nanoparticles1. The appearance of distinct faint brown colour after exposure indicates the intracellular formation of silver nanoparticles12. It gives maximum absorption at 450 nm12. This resonance is clearly missing in the biomass of unexposed vertcillium and the filtrate. Further evidence of intracellular formation is provided by SEM12. The nanoparticles produced after this procedure range between 25+12 nm. These are highly stable in form . The use of fungi is potentially increasing since they secrete large amounts of enzymes and are simpler to deal within laboratory. Application of nanoparticles needs extraction of these particles from their source. This study is concerned...
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