Shigella Flexneri

Topics: Bacteria, Shigella, Microbiology Pages: 2 (494 words) Published: April 30, 2013
Results for Shigella flexneri:
Test | Results | Interpretation | Gram stain| Red; small bacilli with no arrangement| Gram-negative bacillus| 3% KOH| Positive for stringing| Verifies Gram-negative|

Vancomycin | No zone of inhibition| Gram-negative (vancomycin resistant)| Motility | Only the length of stub is red | Negative for motility | Indole| Yellow layer after kovac’s reagent added| Negative for tryptophanase; Indole negative | Methyl Red | Turns red| Positive for acid at pH 4.0 or less ;MR positive| Voges-Proskauer| Stays yellow| Negative for acetoin; VP negative| Citrate Utilization| No growth, Stays green| Negative for citrate permease| Ornithine Decarboxylase | Yellow, negative | Decarboxylase enzyme absent, Only glucose fermented to acid| Lysine Decarboxylase| Yellow ,negative | Only glucose fermented to aid | Triple Sugar Iron(TSI)| Pink slant /yellow but | Glucose fermented to acid and protein in media was broken down to alkaline end product|

Shigella is a genus of gamma proteobacteria in the family Enterobacteriaceae. Shigellae are Gram-negative, nonmotile, non-spore forming, rod-shaped bacteria, very closely related to Escherichia Shigella were discovered over 100 years ago by the Japanese microbiologist, Shiga, for whom the genus is named. There are four species of Shigella: S. boydii, S. dysenteriae, S. flexneri, and S. sonnei. Shigella sonnei, also known as Group D Shigella, accounts for over two-thirds of the shigellosis in the United States. Shigella flexneri, or Group B Shigella, accounts for almost all of the rest. Other types of Shigella are rare in this country, although they are important causes of disease in the developing world. One type, Shigella dysenteriae type 1, causes deadly epidemics in many developing regions and nations. coli. Shigella flexneri is aleading cause of infant mortality in developing countries Shigella are...
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