SCI250 Week 1 Chapter 3 Quiz

Topics: Staining, Bacteria, Gram staining Pages: 5 (756 words) Published: April 2, 2015

SCI250 Week 1 Chapter 3 Staining Lab Quiz

Section: Multiple Choice

1. Which of the following stains is used frequently to identify Mycobacterium and other bacteria whose cell walls contain high amounts of lipids? A. Gram stain
B. Schaeffer-Fulton stain
C. Acid-fast stain
D. Lipidialar stain
E. Spore Stain
2. Which of the following stains is used to classify microorganisms based on their cell wall content? A. Capsular stain
B. Gram stain
C. Spore stain
D. Negative stain
E. Methylene blue
3. In the Gram stain, Gram-positive bacteria stain with a _________ color due to the incorporation of the _________ dye into their cell wall. A. purple, safranin
B. purple, crystal violet
C. red, safranin
D. red, crystal violet
4. What is the function of the mordant iodine in a Gram staining procedure? A. A mordant causes the crystal violet to run.
B. A mordant fixes the bacteria to the slide.
C. A mordant keeps dye attached to an object.
D. All of the above
5. What is the order of reagents used in the Gram stain?
A. crystal violet, iodine, safranin, alcohol
B. alcohol, crystal violet, iodine, safranin
C. iodine, crystal violet, safranin, alcohol
D. crystal violet, iodine, alcohol, safranin
E. crystal violet, safranin, alcohol, iodine
6. Following a Gram stain you observe clear, glassy areas inside the bacterial cells. What does this suggest? Which stain would you use to confirm this? A. A simple stain
B. A flagellar stain
C. Another Gram stain
D. An endospore stain
E. An acid-fast stain
7. The presence of a capsule around bacterial cells usually indicates their increased disease-causing potential and resistance to disinfection. Capsules are generally viewed by ________ A. spore staining.

B. scanning electron microscopy.
C. Gram staining.
D. Ziehl-Neelsen staining.
E. negative staining.
Section: True or False

8. Fluorescent microscopes use an ultraviolet (UV) light source and fluorochromes as stains. True
False
9. Older, pure cultures of Gram-positive organisms can appear red when Gram stained. True
False
Section: Matching

10. Use of two or more dyes to differentiate among bacterial species or to distinguish various structures of an organism; for example, the Gram stain. A. Differential stain
B. Flagellar stain
C. Gram stain
D. Negative stain
E. Schaeffer-Fulton spore stain
F. Simple stain
G. Ziehl-Neelsen acid-fast stain
H. Cationic dye (also called basic dye)
11. A technique for observing flagella by coating the surfaces of flagella with a dye or a metal such as silver. A. Differential stain
B. Flagellar stain
C. Gram stain
D. Negative stain
E. Schaeffer-Fulton spore stain
F. Simple stain
G. Ziehl-Neelsen acid-fast stai
H. Cationic dye (also called basic dye)
12. A differential stain that uses crystal violet, iodine, alcohol, and safranin to differentiate bacteria. Positive bacteria stain dark purple; negative ones stain pink or red. A. Differential stain

B. Flagellar stain
C. Gram stain
D. Negative stain
E. Schaeffer-Fulton spore stain
F. Simple stain
G. Ziehl-Neelsen acid-fast stain
H. Cationic dye (also called basic dye)
13. Technique of staining the background around a specimen, leaving the specimen clear and unstained. A. Differential stain
B. Flagellar stain
C. Gram stain
D. Negative stain
E. Schaeffer-Fulton spore stain
F. Simple stain
G. Ziehl-Neelsen acid-fast stain
H. Cationic dye (also called basic dye)
14. A differential stain used to make endospores easier to visualize. A. Differential stain
B. Flagellar stain
C. Gram stain
D. Negative stain
E. Schaeffer-Fulton spore stain
F. Simple stain
G. Ziehl-Neelsen acid-fast stain
H. Cationic dye (also called basic dye)
15. A single dye used to reveal basic cell shapes and arrangements. A. Differential stain
B. Flagellar stain
C. Gram stain
D. Negative stain
E. Schaeffer-Fulton spore stain
F. Simple stain
G. Ziehl-Neelsen acid-fast stain
H. Cationic dye (also called...
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