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Bulletin of Entomological Research (2007) 97, 299–308

doi:10.1017/S000748530700507X

Genetic variation among Mediterranean populations of Sesamia nonagrioides
(Lepidoptera: Noctuidae) as revealed by
RFLP mtDNA analysis
J.T. Margaritopoulos1 *, B. Gotosopoulos1, Z. Mamuris2,
P.J. Skouras1, K.C. Voudouris1, N. Bacandritsos3,
A.A. Fantinou4 and J.A. Tsitsipis1
1

Laboratory of Entomology and Agricultural Zoology, Department of
Agriculture, Crop Production and Rural Environment, University of
Thessaly, Fytokou Str., 38446 Nea Ionia, Magnesia, Greece: 2Department of
Biochemistry and Biotechnology, University of Thessaly, Ploutonos 26,
41221 Larissa, Greece: 3Institute of Veterinary Research of Athens,
Neapoleos 25, Agia Paraskevi, 15310 Athens, Greece: 4Laboratory of
Ecology and Environmental Sciences, Agricultural University of Athens,
Iera Odos 75, 11855 Athens, Greece
Abstract
Restriction fragment length polymorphism analysis of two segments of mitochondrial DNA (COI and 16S rRNA) was used to examine genetic variation
`
in Sesamia nonagrioides (Lefebvre) populations from the Mediterranean basin.
Four populations were collected from central and southern Greece, and five from northern latitudes: Greece, Italy, France and Spain. No variation was observed in
COI, while 16S rRNA segment proved highly polymorphic and 28 different haplotypes were found. Lower intra-population polymorphism was found in the northern populations than in southern ones. Although no significant isolation by distance was found, the UPGMA tree based on Nei’s raw number of nucleotide differences separated the populations into two major groups, i.e. one with the northern (40.6 N–43.4 N) and the other with the southern populations
(37.3 N–39.2 N). Analysis of molecular variance revealed that most of the variation was between the two major groups (WCT = 0.559), and all pairwise comparisons between the northern and southern populations resulted in high and



References: Aris-Brosou, S. & Excoffier, L. (1996) The impact of population expansion and mutation rate heterogeneity on DNA Armstrong, K.A. & Wratten, S.D. (1996) The use of DNA analysis and the polymerase chain reaction in the study of Arnason, U., Gullberg, A. & Widegren, B. (1991) The complete nucleotide sequence of the mitochondrial DNA of the fin Avise, J.C. (1994) Molecular markers, natural history and evolution. Avise, J.C. (2000) Phylogeography. The history and formation of species Bandelt, H.-J., Foster, P., Sykes, B.C. & Richards, M.B. (1995) Mitochondrial portraits of human populations Coates, B.S., Sumerford, D.V. & Hellmich, R.L. (2004) Geographic and voltinism differentiation among North American Ostrinia nubilalis (European corn borer) mitochondrial cytochrome c oxidase haplotypes Cooper, S.J.B., Ibrahim, K.M. & Hewitt, G.M. (1995) Postglacial expansion and genome subdivision in the European Downie, D.A. (2002) Locating the sources of an invasive pest, grape phylloxera, using a mitochondrial DNA gene genealogy. Molecular Ecology 11, 2013–2026. Eizaguirre, M., Lopez, C. & Albajes, R. (2004) Dispersal capacity in the Mediterranean corn borer, Sesamia nonagrioides Excoffier, L., Smouse, P.E. & Quattro, J.M. (1992) Analysis of molecular variance inferred from metric distances among Excoffier, L., Laval, G. & Schneider, S. (2005) Arlequin ver. 3.0: an integrated software package for population genetics Felsenstein, J. (1993) PHYLIP: (phylogeny inference package) version 3.5c Galichet, P.F. (1982) Hibernation d’une population de Sesamia nonagrioides Lef Goudet, J. (1995) FSTAT (vers. 1.2): a computer program to calculate F-statistics Goudet, J. (2001) FSTAT, a program to estimate and test gene diversities and fixation indices (version 2.9.3). html. Updated from Goudet (1995). Harrison, R.G. (1989) Animal mitochondrial DNA as a genetic marker in population and evolutionary biology Kingman, J.F.C. (1980) Mathematics of genetic diversity. 70 pp. Kourti, A. (2007) Mitochondrial DNA restriction map and cytochrome c oxidase subunit I and II sequence divergence

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