Cellular respiration is the process of breaking down organic compounds to create usable energy for plants and animals. Energy that results from this metabolic process is stored in the form of ATP (adenosine triphosphate) but carbon dioxide and water are also end products of this reaction. This makes it possible to study the amount of respiration of a plant or animal by measuring the rate at which carbon dioxide is released by the organism. In this experiment, crickets and germinating seeds will be tested at three different temperature ranges and the carbon dioxide output measured and compared.
To prepare the test, insert the CO₂ (carbon dioxide) probe into an empty respiration chamber and allow 90 seconds for the probe to warm up. Next, calibrate the CO₂ probe and allow 30 seconds for the CO₂ reading to be calculated and record the base reading. After the base reading has been taken weigh an empty respiration chamber in grams and then place 5 to 10 adult crickets, or 5 to 10 germinating seeds into the respiration chamber. Record the weight once again with both the respiration chamber and the organisms combined. Now, subtract the weight of the empty respiration chamber from the weight of the organisms and the respiration chamber together to determine the mass of the crickets or seeds. Continue to prepare the test by placing the probe snugly onto the respiration chamber and ensure that all other holes are sealed. Begin to measure the CO₂ output in ppm (parts per million) at 10-15° C (ice bath), 20-25° C (room temperature) and 35-40° C (heated water bath.) Allow five minutes for the temperature to stabilize when beginning to test a new temperature range and then proceed to collect data with the CO₂ probe. After a 3 minute period of data collection record the temperature inside the respiration chamber. Find the most linear part of the graph created from the data... [continues]
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