Topics: Bacteria, Escherichia coli, Garlic Pages: 13 (2984 words) Published: January 6, 2013
Valenzuela City Science High School
A. Marcelo St. Dalandanan, Valenzuela City

This investigatory project entitled

Comparative Study on the Antibacterial Assays of

Kamias (Averrhoa bilimbi) Leaf Extract and

Garlic (Allium sativum) Bulb Extract

against Escherichia coli

Is submitted to
Mrs. Merlyn M. Gamboa

In partial fulfillment of the requirements in Chemistry

Submitted by:
Del Rosario, Lea Joy
Salih, Chandler Jay
Serrano, Ayra Mari
Sumayang, Noel
Taguinod, Joshua Cedric
III – Meyer

January 7, 2013
Chapter 1
Background of the Study

Escherichia coli is a Gram-negative, rod-shaped bacterium that is commonly found in the lower intestine of warm-blooded organisms (endoderm’s). Most E. coli strains are harmless, but some serotypes can cause serious food poisoning in humans, and are occasionally responsible for product recalls. The harmless strains are part of the normal flora of the gut, and can benefit their hosts by producing vitamin K2, and by preventing the establishment of pathogenic bacteria within the intestine. It causes illnesses like typhoid fever, paratyphoid fever, and food borne.

This strain of bacteria, according to studies and research, is confirmed to be inhibited by organic compounds produced and contained in extracts of kamias leaves and garlic bulbs. These two organic products are commonly used in adding flavor to food and they both exhibit their antibacterial properties on the same strain of bacteria. So, this study aims to compare the observable characteristics of the extracts and the statistical values of the effectiveness and rates of inhibition of the extracts produced from the two organic products against E. coli.

Statement of the Problem

Main Problem:

This study aims to compare the effectiveness of kamias leaf extract and garlic extract as antibacterial agents against Escherichia coli.

Specific Problem:

To prove or disprove that the kamias extract and garlic extract have antibacterial properties against E. coli

To compare and contrast the physical attributes of the two extracts

To compare and contrast the statistical values of the rates of inhibition of the two extracts



The kamias leaf extract has greater effectiveness of its antibacterial property against E.coli compared with garlic bulb extract.

The garlic bulb extract has greater effectiveness of its antibacterial property against E.coli compared with kamias leaf extract.


The kamias leaf and garlic bulb extracts have equal statistical values in the effectiveness of its antibacterial property against E. coli.

The kamias leaf and garlic bulb extracts are both not effective antibacterial agents against E. coli.

Significance of the Study

Kamias is a fruit-bearing tree of the genus Averrhoa, family Oxalidaceae. It was originated in Indonesia, but now, are cultivated and found throughout Philippines, Indonesia, Sri Lanka, Bangladesh, Myanmar, and Malaysia. In Philippines, kamias leaves was being used as a alternative paste on itches, swelling, rheumatism, mumps or skin eruptions. Also it is used on bites of poisonous creatures. Malaysians use fermented or fresh bilimbi leaves to treat venereal diseases. In French Guiana, syrup made from the fruit is used to treat inflammatory conditions. To date there is no scientific evidence to confirm effectiveness for such uses. In India, the fruit of this plant in folk medicine to control obesity.

Garlic is a species of onion genus, Allium. For health benefits, garlic is known as “cure all’ food because it prevents us from most of the diseases or you can say almost all the diseases. Garlic is used to improve immune system of the body. Also it can cure colds and heart diseases. It helps to...
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