Protein Mediated Generation of Human Induced Pluripotent Cells for Maximum Efficiency
Protein Mediated Generation of Human Induced Pluripotent Cells for Maximum Efficiency
Project ID: 3244K
Abstract
Inefficiency of IPS cell generation is a major obstacle in the development of IPS based technologies, such as their use in regenerative medicine. Current methods are limited to approximately 20% efficiency and often come with a trade off in safety. We propose a new technology that would increase the rate of IPS reprogramming to nearly 100%. This technology entails the usage of protein complexes in tandem with small mimicking chemical compounds to achieve this objective. The protein complex is bonded to elements that guide it to a universal nuclear antigen while the small mimicking compounds mimicked the intranuclear signaling of natural proteins responsible for inducing pluripotency.
Protein Mediated Generation of Human Induced Pluripotent Cells for Maximum Efficiency
Project ID: 3244K
Protein Mediated Generation of Human Induced Pluripotent Cells for Maximum Efficiency
Present Technology
Induced pluripotent stem cells (IPS) are pluripotent cells created from non-pluripotent cells. Pluripotent stem cells are cells that are able to differentiate into different kinds of cells and can divide indefinitely, and include embryonic stem cells. In fact, IPS cells are nearly equivalent to embryonic stem cells in form and function, having similar morphology, colony formation patterns, gene expression, and in vivo differentiation patterns. It was even found in 2007 that mouse IPS cells could be used to form viable chimeras. [3] That is, when IPS cells were injected into developing mouse embryos, the embryo developed in vivo and its live birth was successful.
Further studies show that such chimeras are able to mate with normal mice and also produce viable offspring. [4]
Several different methods have been developed to derive IPS cells from 2006 until now.
The original method utilized by the Kyoto team in 2006 was the use of a
Project ID: 3244K
Abstract
Inefficiency of IPS cell generation is a major obstacle in the development of IPS based technologies, such as their use in regenerative medicine. Current methods are limited to approximately 20% efficiency and often come with a trade off in safety. We propose a new technology that would increase the rate of IPS reprogramming to nearly 100%. This technology entails the usage of protein complexes in tandem with small mimicking chemical compounds to achieve this objective. The protein complex is bonded to elements that guide it to a universal nuclear antigen while the small mimicking compounds mimicked the intranuclear signaling of natural proteins responsible for inducing pluripotency.
Protein Mediated Generation of Human Induced Pluripotent Cells for Maximum Efficiency
Project ID: 3244K
Protein Mediated Generation of Human Induced Pluripotent Cells for Maximum Efficiency
Present Technology
Induced pluripotent stem cells (IPS) are pluripotent cells created from non-pluripotent cells. Pluripotent stem cells are cells that are able to differentiate into different kinds of cells and can divide indefinitely, and include embryonic stem cells. In fact, IPS cells are nearly equivalent to embryonic stem cells in form and function, having similar morphology, colony formation patterns, gene expression, and in vivo differentiation patterns. It was even found in 2007 that mouse IPS cells could be used to form viable chimeras. [3] That is, when IPS cells were injected into developing mouse embryos, the embryo developed in vivo and its live birth was successful.
Further studies show that such chimeras are able to mate with normal mice and also produce viable offspring. [4]
Several different methods have been developed to derive IPS cells from 2006 until now.
The original method utilized by the Kyoto team in 2006 was the use of a
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