Prevalence and Molecular Characteristics of Vibrio Cholerae and Escherichia Coli Specific Bacteriophages in Surface Water Samples of Dhaka City

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Prevalence and Molecular Characteristics of Vibrio Cholerae and Escherichia coli Specific Bacteriophages in Surface Water of Dhaka City

A DISSERTATION SUBMITTED TO THE UNIVERSITY OF DHAKA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE IN GENETIC ENGINEERING AND BIOTECHNOLOGY

Submitted by
EXAMINATION ROLL NO. Curzon Hall 402 SESSION: 2010- 2011
REG. NO. HA-919

ABID AL REZA

DEPARTMENT OF GENETIC ENGINEERING AND BIOTECHNOLOGY FACULTY OF BIOLOGICAL SCIENCE UNIVERSITY OF DHAKA DHAKA BANGLADESH

SEMINAR’S COPY

SUPERVISOR’S COPY

EXAMINER’S COPY

PERSONAL COPY

Dedicated To
GEB 7th Batch

Acknowledgement
I have the honor to express my heartfelt thanks and earnest gratitude to my honorable teacher and supervisor Dr. JESMIN, Professor, Department of Genetic Engineering and Biotechnology, University of Dhaka, for her enthusiastic guidance, affectionate inspiration, passionate supervision and endearing company. I owe him a debt of gratitude for her invaluable advice, indispensable cooperation and constructive criticism throughout my thesis work and during preparation of this dissertation. I feel proud to express my heartfelt gratitude to my reverend research supervisor, Dr. S. M. Faruque, Senior Scientist, Molecular Genetics Laboratory, Laboratory Science Division, ICDDR,B, for allowing me to use the facilities and for his valuable suggestion to carry out the work during my thesis under his supervision. I feel greatly honored to have the opportunity of having a scientist like him as my supervisor. I would like to convey my gratitude to Kazi shafi Ahmed for his encouragement and valuable suggestions. My cordial thanks to Shohag bhai, Mumin bhai, Rocy bhai, and especially Nayeem bhai for their continuous advice, encouragement and proper guidance. It might be almost impossible form my part to complete this thesis work without their supreme help. I would like to express my special appreciation to Afjal bhai for his help, suggestion and delightful company. I am thankful to Sajib bhai, Wohid bhai and Farid bhai for their assistance. I would like to thank Rabbani, Arafat, Lamyah, Alvee, Shadlee and Khalid for their help and delightful company. Finally, I want to express my utmost gratitude to my parents and my brothers for their unremitting support.

The Author Department of Genetic Engineering and Biotechnology University of Dhaka

29th July, 2012

Abstract

ABSTRACT
Diarrhea and Cholera are both major health problems in developing countries like Bangladesh where cholera epidemics occur twice a year in a regular seasonal pattern. Studies of E. coli phage and Vibrio phages have been of historical interest and with the rise of drug resistant bacteria; the therapeutic potential of phages has received renewed attention. To understand the natural reservoir of E .coli phages and Vibrio phages, studies were carried out on the prevalence of phages in the surface water in and outskirts of Dhaka city, the capital of Bangladesh. The study was carried out during the time of mid-July, 2011 to mid- August, 2011 for E. coli phages and November, 2011 to April, 2012 for Vibrio phages. A total of 160 water samples (128 for Vibrio phages and 32 for E. coli phages) were used in this study. Forty one samples out of 128 water samples (32%) were scored as Vibrio phage positive and all of the thirty two samples (100%) were positive for the presence of E. coli phages. This study showed higher prevalence of Vibrio phages during inter-epidemic time and low during epidemic period which indicated that Vibrio phages might influence cholera epidemics. It was also suggested that environmental surveillance of V. cholerae and their lytic phages in surface water could play an important role in the control of cholera epidemics. Thus phage surveillance may provide an indication of their prevalence. The observation also suggested that the new E .coli phages (EA 1, 3d, 3r, 4, 5, 6, 7, 8 and 9) and...
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