Plant Tissue Culture Techniques

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Plant Tissue Culture

151

Chapter 9 Plant Tissue Culture Techniques
Lorraine Mineo
Department of Biology Lafayette College Easton, Pennsylvania 18042

Lorraine Buzas Mineo (B.S., Muhlenberg College; M.A., Duke University) is a lecturer in the Department of Biology, Lafayette College, and has taught botany since 1978 and supervised the General Biology Laboratories since 1970. Research interests in physiological and forest ecology have culminated in several publications. Other interests include science education methods.

Reprinted from: Mineo, L. 1990. Plant tissue culture techniques. Pages 151-174, in Tested studies for laboratory teaching. Volume 11. (C. A. Goldman, Editor). Proceedings of the Eleventh Workshop/Conference of the Association for Biology Laboratory Education (ABLE), 195 pages. - Copyright policy: http://www.zoo.utoronto.ca/able/volumes/copyright.htm Although the laboratory exercises in ABLE proceedings volumes have been tested and due consideration has been given to safety, individuals performing these exercises must assume all responsibility for risk. The Association for Biology Laboratory Education (ABLE) disclaims any liability with regards to safety in connection with the use of the exercises in its proceedings volumes.

© 1990 Lorraine Mineo 151
Association for Biology Laboratory Education (ABLE) ~ http://www.zoo.utoronto.ca/able

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Plant Tissue Culture

Contents Introduction....................................................................................................................152 Terminology...................................................................................................................152 Laboratory Requirements for Tissue Culture ................................................................153 Demonstration of "in vitro" Morphogenesis and Totipotency of Seedling Explants ....154 Effects of Hormone Balance on Explant Growth and Morphogenesis..........................160 Callus Formation and Multiplication.............................................................................164 Establishment of Suspension Cultures...........................................................................167 Anther Culture ...............................................................................................................167 Acknowledgements........................................................................................................168 Literature Cited ..............................................................................................................169 Appendices A to E .........................................................................................................170 Introduction Plant tissue culture techniques are essential to many types of academic inquiry, as well as to many applied aspects of plant science. In the past, plant tissue culture techniques have been used in academic investigations of totipotency and the roles of hormones in cytodifferentiation and organogenesis. Currently, tissue-cultured plants that have been genetically engineered provide insight into plant molecular biology and gene regulation. Plant tissue culture techniques are also central to innovative areas of applied plant science, including plant biotechnology and agriculture. For example, select plants can be cloned and cultured as suspended cells from which plant products can be harvested. In addition, the management of genetically engineered cells to form transgenic whole plants requires tissue culture procedures; tissue culture methods are also required in the formation of somatic haploid embryos from which homozygous plants can be generated. Thus, tissue culture techniques have been, and still are, prominent in academic and applied plant science. The techniques demonstrated in these exercises range from simple ones that can easily be performed by beginning students to those done by botany or physiology students. Experiment 1 and 2...
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