Placenta Enriched Agar as a Selective Medium for Brucella Spp

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COMPARISON BETWEEN PLACENTA AGAR AND NUTRIENT AGAR ON GROWTH OF Staphylococcus aureus AND Escherichia coli

Chloe Dominique Acero
Kristine Marie Gonzales
Hannah Marie Hermosisima
Patrisha joy Morales
Joanna Keziah Ramos

Group 4
BSMT3E

Background of the Study

Placenta is an organ characteristic of a true mammal during pregnancy, joining mother and offspring, providing endocrine secretion and selective exchange of soluble, but not particulate, blood borne substances through an opposition of uterine and trophoblastic vascularized parts. Although many bacteria may cause the infection of the placenta, the most common organism associated with this septicity includes Staphylococcus aureus, Escherichia coli and Bacteroides.

Staphylococcus aureus and Escherichia coli can grow on a wide range of media. Primary isolation can be made on a Nutrient Agar, Mac Conkey Agar or Blood Agar Plate.
On Nutrient Agar, Staphylococcus aureus produces round, convex, well defined colonies, and measuring 2-4 mm in diameter. The colonies show a buttery consistency with a smooth glistening surface. Staphylococcus aureus produces golden yellow colonies due to production of staphyloxanthin. According to Burgess et al, of the Walter and Eliza Hali Institute of Medical Research: “Human placental conditioned medium is able to stimulate human bone marrow cells to form neutrophilic granulocyte-monocyte colonies and eosinophil colonies in agar cultures.”

The question in everyone’s mind is: Is it possible to grow a gram negative and a gram positive bacteria on an agar supplemented by Placenta.

Methodology
I. Preparing the Placenta
a. Boiling of Placenta (25g, 50g, 100g) in a 500 ml distilled water b. Strain to get the broth
II. Preparation of Placental Agar
c. Mix the broth from a 25 g placenta to 1 teaspoon of agar powder. Do the same to the broth with 50 g and 100 g placenta respectively d....
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