Physioex Acid Base Exercise10

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Exercise 10: Acid/Base Balance Worksheet

Respiratory Acidosis and Alkalosis
Activity 1: Normal Breathing

1. At 20 seconds, pH = 7.38
2. At 40 seconds, pH = 7.38
3. At 60 seconds, pH = 7.38
4. Did the pH level of the blood change at all during normal breathing? If so, how? No. 5. Was the pH level always within the “normal” range for the human body? Yes. 6. Did the PCO2 level change during the course of normal breathing? If so, how? No.

Activity 2a: Hyperventilation – Run 1

1. At 20 seconds, pH = 7.45
2. At 40 seconds, pH = 7.5
3. At 60 seconds, pH = 7.66
4. Maximum pH =
5. Did the pH level of the blood change at all during this run? If so, how? Yes. pH increased and decreased significantly. 6. Was the pH level always within the “normal” range for the human body? If not, when was the pH value outside of the normal range, and what acid/base imbalance did this pH value indicate? No. pH was outside the normal range at around 5 seconds after hyperventilation started. The acid/base imbalance indicated by the pH value was respitory alkalosis 7. Did the PCO2 level change during the curse of this run? If so, how? Yes. Progressively dropped. 8. If you observed an acid/base imbalance during this run, how would you expect to renal system to compensate for this condition? Hypoventilation causes an increase in Pco2, generation of H+ causes a decrease in plasma pH, secretion of HCO3- into urine causes a decrease in plasma pH. 9. How did the hyperventilation trace differ from the trace for the normal breathing? Did the tidal volumes change? The hyperventilation trace went to higher and lower extremes than the trace for normal breathing. Yes 10. What might cause a person to hyperventilate? Traveling to a higher altitude, fever, and anxiety.

Activity 2b: Hyperventilation – Run 2

1. What happened to the trace after the 20-second mark when you stopped the hyperventilation? Did the breathing return to normal immediately? Explain your observation. Tidal volume began to stabilize. No. Trace almost flat lined for about 10 seconds before normalizing.

Activity 3: Rebreathing

1. At 20 seconds, pH = 7.34
2. At 40 seconds, pH = 7.3
3. At 60 seconds, pH = 7.26
4. Did the pH level of the blood change at all during this run? If so, how? Yes. Dropped progressively below normal value. 5. Was the pH level always within the “normal” range for the human body? If not, when was the pH value outside of the normal range, and what acid/base imbalance did this pH value indicate? No. After 10 seconds of rebreathing. Respitory acidosis. 6. Did the PCO2 level change during the course of this run? If so, how? Yes. Progressively increased. 7. If you observed an acid/base imbalance during this run, how would you expect the renal system to compensate for this condition? Hyperventilation causes a decrease in Pco2, generation of HCO3- causes an increase in plasma pH, secretion of H+ into urine cases an increase in plasma pH. 8. How did the rebreathing trace differ from the trace for normal breathing? Did the tidal volumes change? Trace value dropped lower than trace value for normal breathing. Yes. 9. Give examples of respiratory problems that would result in pH and PCO2 patterns similar to what you observed during rebreathing? Airway obstruction, depression of the respiratory center in the brain stem, lung disease, and drug overdose.

Renal System Compensation
Activity 4: Renal Response to Normal Acid/Base Balance

1. At normal PCO2 and pH levels, what level of H+ was present in the urine? Normal 2. What level of [HCO3-] was present in the urine? Normal 3. Why does the blood pH value change as PCO2 changes? Carbon dioxide levels have a direct effect on pH levels because the addition of carbon dioxide to the blood results in the generation of more H+. 4. How does the blood pH value change as Pco2 changes? Inversely. If Pco2 goes up...
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