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Dela Cruz, Catherine V.Genetics and Evolution Prof. Genelita Garcia Obio, Mary Joy A. Written Report
Stem Cell Technology
Stem Cells – cells that have the ability to continuously divide and differentiate into various other kind of cells. Characteristics:
* Simple, remain the same cell in constant environment
* Continuous mitotic divisions
* With proper stimuli/environment it can differentiate into various cells Classification:
Type of cells| Description| Source|
Totipotent| Can give rise to a whole organism| Fertilized egg cell| Pluripotent| Can give rise to any cell but not an entire organism| Embryonic stem cells| Multipotent| Can give rise to cells within the same lineage| Adult stem cells| * According to potency

* According to sources
Embryonic – stem cells that can be collected in the inner mass of the blastula. Adult - Stem cells reside in a microenvironment known as the niche. Schofield, who was the first to put forth the concept of stem cell niche, proposed that the niche: (1) provides an anatomic space to regulate stem cell number, (2) instructs a stem cell to either self-renew in close proximity or commit to differentiation at a distance, and (3) influences stem cell motility. Adult| Location (niche)| Stem cells| Growth factor| Give rise to| Bone| Bone marrow-soft, spongy tissue found inside bones. It is the medium for development and storage of most of the body's blood cells.| Hematopoetic cells| (G-CSF) granulocyte- colony stimulating factor| Blood cells| Blood| | Hematopoetic cells| (G-CSF) granulocyte- colony stimulating factor| Blood cells| Teeth| Pulp of teeth| | | |

Brain| Hippocampus-located within the temporal lobes, adjacent to the amygdala.- involved in memory forming, organizing, and storing.| Neural stem cells| NGF [Nerve Growth Factor]| NeuronsAstrocytesOligodendrytes| Skin| Basal layer of epidermis?Basal layer of hair follicle| Follicular stem cells| EGF [Epidermal Growth Factor| Hair folliclesepidermis| Adipose| | | | |

Intestine| Crypts of intestine| Epithelial stem cells| | Absorptive cellsGoblet cellsPanethEnteroendocrine| * Manner of transplant
Allogeneic – stem cells came from family / unrelated donor Autologous – self-donation
Syngeneic – identical twin
Xenogeneic – other species (ex. sheep)
Procedure
A. Adult Stem Cell Harvest
ADIPOSE STEM CELL HARVEST
Fats are harvested in the abdomen or waist through small incision using the process of mini liposuction.

Fats are harvested in the abdomen or waist through small incision using the process of mini liposuction.

For stem cells proliferation
For stem cells proliferation
Get sample and place in another test tube then centrifuge.

Get sample and place in another test tube then centrifuge.

Fats are place in the test tube for the addition of cell culture product.

Fats are place in the test tube for the addition of cell culture product.

Separates dormant stem cells from the fat
Separates dormant stem cells from the fat
Abundant cell availability from discarded tissues
Abundant cell availability from discarded tissues

For the activated stem cells go with the circulation of the blood For the activated stem cells go with the circulation of the blood Add activated stem cells to drip saline bag and returned to patient by IV drip

Add activated stem cells to drip saline bag and returned to patient by IV drip

Activate the dormant stem cells
Activate the dormant stem cells
Photo activation of dormant stem cells

Photo activation of dormant stem cells

This makes the stem cells easy to locate or attract to the injury

This makes the stem cells easy to locate or attract to the injury

Add PRP to dormant stem cells.

Add PRP to dormant stem cells.

Separates the platelet rich plasma to other components of the blood Separates the platelet rich plasma to other components of the blood Get small...
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