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Biol. 412 - Bacteriology

Growth! The Ultimate Goal
Required readings in Madigan et. al.:
pp. 118-132 (13th) or 142-157 (12th) pp. 86-90 (13th) or 108-113 (12th )
pp. 143-147 (13th) or 168-172 (12th) Optional Moodle readings -1. Beyond Binary Fission and 2.Measuring Growth in Soil A. The Basics of Microbial Growth - REVIEW LAB NOTES on growth. Lecture will build on the concepts covered in lab lectures. 1. Individuals vs. populations –

Binary fission – the most common mode of individual growth
role of tubulin and actin homologues in growth/shape of individuals Viviparity – another example of how bacteria may grow.
2. Role of the cytoskeleton IN GROWTH OF INDIVIDUALS
3. Plotting growth of POPULATIONS to determine generation time (aka doubling time). Phases of growth - REVIEW LAB LECTURE NOTES -what are they and what do they tell you? Growth rates and generation times N = N02n

n= 3.3 (log N - log N0)
determine it graphically
4. Batch cultures vs. Chemostats - What are they?
When do you use one vs. other?
How would you expect physiology to differ for organisms growing in one vs. other? 5. growth in cultures vs. the real do they differ?
growth nutrients oxygen

Biol. 412 - Bacteriology
B. How we measure growth of populations. REVIEW LAB LECTURE NOTES 1. Direct Counts = microscopic counts How do you do them?
2. Viable Counts = plate counts How do you do them?
advantages disadvantages
3. Turbidity
How does it work?
C. The specifics of growth requirements - 1. What a cell needs to make? An E. coli cell only weighs 9.5 x 10-13 g (about 70% of this is water) approximately...... 55% dry weight = protein (~1050 different kinds) 20.5% dry weight RNA (81% = rRNA)
3% dw DNA
9.1% dw lipid 3.4%dw lipopolysaccharide
2.5% dw murein (aka peptidoglycan)
2.5% dw glycogen
2.9% dw soluble metabolites, building blocks, vitamins, etc 1% inorganic ions 2...
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