Osmosis and Diffusion Lab

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Osmosis and Diffusion

Title: Diffusion and Osmosis AP Lab 1

Purpose: The purpose of this activity is to determine the effects of a selectively permeable membrane on diffusion and osmosis between two solutions separated by a membrane. Hypothesis:
Procedures: In lab manual
Material: In lab manual
Background Information:
Data:

Table 1
Diffusion of glucose and iodine.

| Color| Glucose|
Time| Dialysis Bag| Beaker| Dialysis Bag| Beaker|
Start| | | | |
30 minutes| | | | |

Table 2
Osmosis Investigation – Water gain by dialysis bags

Solution| Dialysis BagInitial Mass (g)| Dialysis BagFinal Mass (g)| Change in Mass (g)| % Change in Mass| Water| | | | |
0.2 M| | | | |
0.4 M| | | | |
0.6 M| | | | |
0.8 M| | | | |
1.0 M| | | | |

Table 3
Potato Cell Water Potential- Gain and loss of water in different environments.

| Solution Temperature | Potato Cylinder|
Solution| Initial Temp| Final Temp| Initial Mass (g)| Final Mass (g)| Change in Mass (g)| % Change in Mass| Water| | | | | | |
0.2 M| | | | | | |
0.4 M| | | | | | |
0.6 M| | | | | | |
0.8 M| | | | | | |
1.0 M| | | | | | |

Plant Cell Plasmolysis

Cells in Distilled Water
|

Cells in 15% NaCl

|

Conclusion:
1.       Create a Venn diagram comparing osmosis and diffusion. 2.       Part A of the experiment was a demonstration of diffusion. Give an example of diffusion occurring in the setup. Do you think osmosis occurred in this part of the experiment? If yes, explain why you believe this to be. 3.       Did the dialysis tubing serve as a selectively permeable membrane? Explain your answer. 4.       In part B, what caused the mass of the dialysis bags to change? Was there more or less water in the dialysis bags at the conclusion of the experiment? Explain. 5.       Was the distilled water in the beakers hypertonic or hypotonic in relation to...
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