Organic Chemistry Lab Report

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Brooke BeamsFebruary 2, 2012
Experiment 1
Determination of the Components of a “Mixed Drink”

INTRODUCTION. For this experiment standard solutions of Kool-Aid flavors will be prepared to measure the absorbance at lambda max and plot the results against the concentration of solution to generate a standard curve. Then it must be determined the amount of each flavor in a sample of the unknown by comparison to your standard curves. The ingredient in each flavor of the Kool-Aid that was tested is Blue 1 and Red 40. The Lambda max for red 40 is 503nm and for the blue 1 it is 629nm. A UV/Vis spectrophotometer was used to measure the absorbance of the Cherry, Berry Blue, and unknown II.

The purpose of this experiment is to determine the amount of each flavor in the unknown.

PROCEDURE.1 Using wax paper, 0.4003 g of Berry Blue Kool-Aid was measured out on the analytical balance and added to a 100 ml volumetric flask. Then distilled water was added to fill the flask to the volume mark. The flask was stoppered and mixed thoroughly. Using a volumetric pipette 1.00, 2.00, 3.00, 4.00, 5.00, and 6.00 ml of solution was added to six different clean and dry test tubes. To each of those test tubes was added enough distilled water to equal a total of 6.00 ml of solution in each test tube. The absorbance of each solution was taken at its lambda max of 629nm and recorded.

For the cherry flavor, using wax paper, 0.1109 g of Kool-Aid was measured out and the steps were repeated. The absorbance of each solution was taken at both lambda max of 503nm and 629nm because the cherry flavor contains both the blue 1 and red 40.

The absorbance of the unknown II given was measured and recorded. The unknown was then divided into 5 clean and dry test tubes and filled with 1.00, 2.00, 3.00, 4.00, and 5.00 ml of solutions and diluted with distilled water to equal 6.00 ml of solution in each test tube. The absorbance of each of these were measured at both...
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