Oral Glucose Tolerance Test

Topics: Glucose tolerance test, Blood sugar, Diabetes mellitus Pages: 3 (1179 words) Published: July 17, 2008
Experimental Purpose
The purpose of the Variables in Glucose Tolerance lab was to examine the changes in blood sugar during glucose tolerance test conducted with test rats. We will be using three rats and perform the Oral Glucose Tolerance Test to measure the glucose levels in the rats blood. The oral glucose tolerance test is a laboratory method to check how the body breaks down (metabolizes) blood sugar. Glucose is the sugar that the body uses for energy. Patients with untreated diabetes have high blood glucose levels. Glucose tolerance tests are one of the tools used to diagnose diabetes. (Hypothysis) Materials ad Methods

The independent project is a group project in which the class in split into groups of 4-5. My group consisted of James, Jaime, Omar, Kirk, and Myself. We selected our two drugs which were Glyberide and Insulin. We discussed who would be doing what on the day of the project and how we would manage our time. I had to record all of the data that we received form our experiment and observe the behavior patterns that rats displayed inside their plastic restraint. Omar was responsible for researching the affects of glucose; James was responsible for researching the affects of insulin, and Kirk and Jamie were responsible for researching the affects of glyberide. The materials that were used in the independent project were 3 male rats, one touch glucometer and strips, gloves, syringes, scissors, orange marker, plastic rat restraint, and a stop watch to conduct our project. We selected three rats and marked their tails with orange sharpie in order to differentiate between the rats when we conduct the actual project. We weighted each of the rats to ensure we give accurate amounts of each drug. Previous to selecting the rats, the food was removed form his cage for approximately 24 hours so the rats could undergo a fasting cycle. The three drugs we used were Glucose, Insulin, and Glyberide. When determining how much of each drug to inject into the...
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