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Molecular Biology

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Molecular Biology I Pre-lab Assignment:

Before completing this assignment, read the Molecular Biology I and II Lab Exercise posted on CourseWeb. You should not re-type the questions, but the answers must be typed and written in complete sentences. You will turn in a hard copy to your instructor and submit an electronic copy to Turnitin.com using the enrollment password pittbio2013 and the class ID provided by your instructor. Directions for submitting an assignment to Turnitin.com are posted on CourseWeb.

This assignment must be completed independently. NO GROUP WORK. Failure to follow this direction will result in penalty for violation of academic integrity.

1. What is a plasmid? How is it different from genomic or chromosomal DNA?
2. What does the term “transformation” mean as it applies to molecular biology? Are YOU transforming the E. coli?

3. E. coli cells that contain pAMP will be able to grow on which of the following LB-agar plates – LB-KAN, LB-AMP, and/or LB-AMP/ARA? Explain how the plasmid permits E. coli cells to grow on the plate(s).

4. E. coli cells that contain pKAN will be able to grow on which of the following LB-agar plates – LB-KAN, LB-AMP, and/or LB-AMP/ARA? Explain how the plasmid permits E. coli cells to grow on the plate(s).

5. E. coli cells that contain pGLO will be able to grow on which of the following LB-agar plates – LB-KAN, LB-AMP, and/or LB-AMP/ARA? On which plate(s) will cells low in the presence of UV light? Explain how the plasmid permits E. coli cells to glow and/or grow on the plate(s).

6. Restriction enzymes cut each plasmid into fragments of known base pair (bp) lengths. These are the "expected" base pair lengths. Use the plasmid maps on page 3 of the lab exercise (Figure 1) to determine the "expected" base pair lengths of pAMP, pKAN, and pGLO DNA after digestion with BamH I and Hind III.

7. Imagine that you digested pKAN, pAMP, and pGLO DNA with BamH I and...