Microscopy Use and Function

Microscopy Use and Function
Hands-On Labs, Inc. Version 42-0089-00-01

Exercise 1: Identifying Parts of a Compound Light
Microscope
Question
A. Study and label the microscope parts in Figure 3.
Microscope Parts

Microscope Parts
A
EYEPIECE
G
MIRROR/ILLUMINATOR
B
TUBE
H
BASE
C
REVOLVING NOSEPIECE/TURRET
I
LONGITUDINAL CONTROL KNOB
D
OBJECTIVE LENS
J
TRANSVERSE CONTROL KNOB
E
STAGE
K
FINE ADJUSTMENT
F
DIAPHRAIGM
L
CASE ADJUSTMENT
Microscopy Use and Function
Exercise 2: Illuminated Pocket Microscope

Questions
A. Draw the image of the root tips as viewed at 200X.

See attached page for actual sketches

B. Label the diagram of the pocket microscope in Figure 4. Use the following terms: eyepiece, stage clips, magnification, focus adjust, light, and light switch.

Pocket Microscope Parts
A
EYEPIECE
B
MAGNIFICATION
C
FOCUS ADJUST
D
LIGHT SWITCH
E
LIGHT
F
STAGE CLIPS
Use and Function
Exercise 3: Total Magnification, Determining Field of View and Depth of Field

Observations
Data Table 1: Magnification Observations

Data Table 1: Magnification Observations

Ocular Lens
Magnification
Objective Lens
Magnification
Total
Magnification
Scanning
15X
4X
60X
Low
15X
10X
150X
High
15X
40X
600X

Data Table 2: Field of View Observations

Data Table 2: Field of View Observations

Total
Magnification

Diameter of Field mm

Diameter of Field µm
Scanning Power
60X
5 mm
5000 µm
Low Power
150X
2 mm
2000 µm
High Power
600X
0.5 mm
500 µm

Exercise 3: Total Magnification, Determining Field of View and Depth of Field

Observations
A. Draw a picture of what the threads look like under the microscope using the following powers of magnification:

See attached page for actual sketches
1. Scanning

2. Low Power

3. High Power

Microscopy Use and Function
B. Draw a picture of what the letter “e” looks like under the following using the following powers of magnification: