1. What is the correct procedure for carrying the microscope?
A microscope is a valuable piece of laboratory instrument, thus it must be handled carefully. Preparation is important when moving a microscope. Make sure that the path to the new destination is clear of obstructions and that the area where the microscope will be placed is clean. Prepare the microscope for transport. If there is a slide under the microscope, remove it. Carefully place one hand on the arm of the microscope. Slowly lift the microscope up, and place your other hand underneath the base to provide support. Walk slowly and keep the microscope close to your body while carrying it to the new destination. Set the microscope down gently in the new location. 2. What is a parfocal microscope?
Parfocal microscope is a lens that stays focus when magnification is changed. For example, if the focal point of a microscope is changed from a low power objective (10x) to a higher power (40x or 43x), the object stays in focus. 3. What is total magnification? How it is computed? Compare the image viewed prepared slides under LPO and HPO?
Magnification is the process of enlarging something only in appearance, not in physical size. Typically, magnification is related to scaling up visuals or images. It does not change the perspective of the image. Total Magnification is determined by multiplying the eyepiece power (usually 10x) by the objective lens in place. The total appearance of the image we viewed was seen under the LPO, and for a more detailed appearance and better resolution, HPO was used.
4. Why it is important not to turn the coarse adjustment knob when focusing using the HPO? Give an instance when it is needed to turn the coarse adjustment know upon focusing using the HPO?
It is significant not to use the coarse adjustment knob in focusing using HPO to avoid damaging or cracking the slide due to the length of the objective. It is advisable to first use the...
Please join StudyMode to read the full document