Micro Biology Lab Report Material

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Melvin Johnson
Unknown Lab #1


There are many reasons for identifying an unknown bacterium.  The reasons range from medical purposes, such as determining if the unknown could cause ailments in living things or knowing what microorganisms are present and what antibiotics are needed. The purpose of an unknown lab is to identify an unknown bacteria culture using a number of differential tests. The tests performed on the unknown bacteria cultures were all used to determine the identity of the bacteria.   Each of the tests performed provided some key information about the bacteria in question and how it function Identification of the unknown cultures was accomplished by sorting out and differentiating possible bacteria based on specific biochemical and morphological characteristics. The tests performed and materials used identified specific enzymatic reactions or metabolic pathways. Each test used helps to recognize the particulars and identify the unknown bacteria. The tests used to identify the unknown cultures were TSIA, SIM’s H2S, Indole, Motility, Glucose fermentation, VP, Citrate, Urease, Gelatin, Nitrate NO3 reduction, Catalase, Casien, and Starch. s.

Physiologically, E. coli is versatile and well-adapted to its characteristic habitats. It can grow in media with glucose as the sole organic constituent. Wild-type E. coli has no growth factor requirements, and metabolically it can transform glucose into all of the macromolecular components that make up the cell. The bacterium can grow in the presence or absence of O2. Under anaerobic conditions it will grow by means of fermentation, producing characteristic "mixed acids and gas" as end products. However, it can also grow by means of anaerobic respiration, since it is able to utilize NO3, NO2 or fumarate as final electron acceptors for respiratory electron transport processes. In part, this adapts E. coli to its intestinal (anaerobic) and its extraintestinal (aerobic...
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