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AS/OCT2010/MIC455

UNIVERSITI TEKNOLOGI MARA FINAL EXAMINATION

COURSE COURSE CODE EXAMINATION TIME

GENERAL MICROBIOLOGY MIC455 OCTOBER 2010 3 HOURS

INSTRUCTIONS TO CANDIDATES 1. 2. This paper consists of ten (10) questions. Answer ALL questions in the Answer Booklet. Start each answer on a new page. Do not bring any material into the examination room unless permission is given by the invigilator. Please check to make sure that this examination pack consists of: i) ii) the Question Paper an Answer Booklet - provided by the Faculty

DO NOT TURN THIS PAGE UNTIL YOU ARE TOLD TO DO SO
This examination paper consists of 5 printed pages
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QUESTION 1 Fill in the blank with the appropriate answers. Write the answers in the answer booklet. a) The two (2) main components making up the peptidoglycan of bacterial cell wall are and . The scientific names of any two (2) microorganisms that are not classified as prokaryotes are and The two (2) genera of bacteria that are capable of producing spores are and

b)

c)

d)

An example of a Gram positive bacteria is example of a Gram negative bacteria.

and

is an

e)

Three (3) common differential staining methods used in microbiology are , and "Ubiquitous" in microbiology means The minimum inhibitory concentration (MIC) means Of more than three thousand (3000) species of bacteria, only 10% cause disease. The three (3) diseases and their causative agents are , and

f) g) h)

i) j)

Bacteriocidal agents are defined as Two (2) methods that can be used to surface sterilize the skin of a human are and An antigen is defined as (10 marks)

k)

QUESTION 2 As a microbiologist, one (1) of the most important skills to develop is the ability to determine the microbial population from any given samples using any logical methods available in microbiology. Using serial dilution plating method, illustrate how would you determine the microbial population from a given 100 ml sewage wastewater sample by using a detail flowchart with your own imaginary data. (10 marks)

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QUESTION 3 a) Living cells, namely, microorganisms can be killed using several modes of action that are targeted against their structure and cellular properties, thus affecting their physiology and wellbeing leading to the death of the microorganisms. Specifically describe all these modes of actions. (5 marks) b) Name one (1) method of sterilization for each of the modes given in your answer for the question above. (IVz marks) c) List all the factors that will affect the rate of death of a microorganism with reference to the use of physical or chemical killing agents. (2Y2 marks) QUESTION 4 The following growth data in table 1 below was obtained when the effect of pH on the growth of Escherichia coli was studied in Nutrient Broth at 35°C. From the data, a) Determine the cell population for both culture at all intervals. Show one (1) example of your calculation. (6 marks) Derive the relevant information that can be concluded from the data. (Show all calculations) (6 marks) Derive relevant conclusion, if any, from the experiment (the conclusion must be relevant to the data obtained from your calculation). (3 marks) Incubation Time (min) 0 20 40 60 80 120 150 E. coli in Nutrient Broth, pH 5 at 35°C. Cell count per 4 hemocytometer squares 4 5 7 8 9 12 15 E. coli in Nutrient Broth, pH 7 at 35°C. Cell count per 4 hemocytometer squares 4 7 15 28 54 100 191

b)

c)

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QUESTION 5 It has been suggested that Koch's postulates are no longer relevant.What are the arguments that contradict each one (1) of the four (4) Koch's postulates. (8 marks)

QUESTION 6 Answer in general on (i) special features and...
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