Limits

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Limits

By | August 2013
Page 1 of 3
Limitations of the study.
This research conducted only on a specific area to compare and isolate bacteria in a money from bank and money from environment like markets, Material and Methods

Samples and Sampling: The study samples were collected based on the level of usage and thus circulation. This was made up of 40 One Ghana Cedi notes (GH¢ 1), 25 Five Ghana Cedi notes (GH¢ 5), 20 Ten Ghana Cedi notes (GH¢ 10), 10 Twenty Ghana Cedi (GH¢ 20) notes and 5 Fifty Ghana Cedi notes (GH¢ 50) collected randomly from sellers on the major streets and markets of the Cape Coast Metropolis into sterile paper bags between September, 2009 to March, 2010 and transported to the Laboratory of the Department of Laboratory Technology, University of Cape Coast for bacteriological analysis on the same day. Four currency notes of each denomination and not in circulation obtained from the Central Bank were used as control samples.

Culture and Isolation of Bacteria: Each currency note was aseptically transferred into individual universal bottles containing 10 ml of sterile buffered peptone water and the bottle vigorously shaken for 2 minutes. The currency is removed and the resulting peptone water solution served as a test sample and incubated for 24hours at of 37oC. The incubated test sample was then cultured onto Blood agar, MacConkey and Cysteine Lactose Electrolyte Deficient (CLED). The plates were incubated aerobically overnight in an incubator at 370C. Pure cultures were obtained by sub-culturing distinct colonies. Control samples underwent the same processes.

Identification of Isolates: Pure isolated colonies were identified using their Morphology, Gram reaction as well as biochemical techniques such as the Indole Catalase, Coagulase, Oxidase, Urease, Catalase test and Triple sugar iron tests (sugar fermentation and gas production).

Statistical Analysis: Data from study was analyzed descriptively using SPSS 16.0 software.

Results

All 100 samples analysed were...