Lima beans are edible flat whitish beans and are good to use for any experiment because they represent the human body in that they both go through all of the same processes of growth and development. This lab uses lima beans in order to determine whether they were viable or not. If a bean is viable, it means that it is alive: able to grow and develop. The two ways to find out whether a bean is viable or not, are a tetrazolium test and germination test. Tetrazolium is a chemical that turns red in the presence of hydrogen ions. All living organisms release hydrogen ions every day and therefore, it was possible to see whether the lima beans were viable or not using tetrazolium. This is a result of metabolic activity. Germination, the other possible test, is the process where beans or spores sprout and begin to grow. Both of these ways test whether the organism has a metabolism or not, which are the chemical processes that occur within a living organism in order to maintain life.
The lima bean viability lab tested for viability in two distinct bean types through a germination and tetrazolium procedure.
Experimental Design: See protocol.
If the lima beans are tested with germination and tetrazolium tests, then 25 beans out of the 25 beans of both types that were tested will be viable because they are all alive.
|TETRAZOLIUM TEST |TOTAL NUMBER OF SEEDS USED |NUMBER OF SEEDS TURNING PINK |% SEEDS SHOWING VIABIITY | |TYPE A | | | | |1 Carolyn and Kelly |25 |25 |100 | |2 Julie and Emily |22 |22 |100 | |3 Annie and Meg |24 |24 |100 | |4 Thomas and Nathan |25 |25 |100 | |5 Mazin andJessie and Robin |25 |25 |100 | |6 Lauren and Henry |25 |25 |100 | |7 Carolyn and Michaela |25 |25 |100 | |8 Ryan ad Bernadette |23 |23 |100 | | | | | | |1 Kaylegh and Shannon |25 |25 |100 | |2 Marissa and Jasmine |25 |25 |100 | |3 Meghan and Nyah and Jennie |25 |25 |100 | |4 Ishani and Morgan |25 |25 |100 | |5 Virginia and Katie |25 |25 |100 | |6 Briana, Carly, and Elizabeth |25 |25 |100 | |7 Alex and Austin |25 |25 |100 | |8 Jimmy and Christina |24 |24 |100 | |9 | |...