Lab Report: Effect of Abiotic and Biotic Factors on Enzymatic Reactions
Ryan Kawiecki
Ms. Lahr
Ap-Biology
September 17, 2013
Title:
The Effect of Abiotic and Biotic Factors on Enzymatic Reactions
Problem:
Will increased pH affect the rate of reaction in an enzyme catalyzed reaction?
Hypothesis:
Increased pH substance will increase the rate of reaction
Table:
Table 1: Baseline of peroxide
Time (min)
Qualitative color
1:00
5
2:00
6
3:00
7
4:00
8
5:00
9
Table 2: effect of increased pH on peroxidase
Increased pH by 5mL
Color intensity
2
10
5
10
1
10
8
9
11
10
3
10 Time in minutes
Graph:
Graph1: The effect of pH on the enzyme Peroxidase
Y- value rate of reaction X- value = pH of solution
Conclusion: This experiment was to test if increased amount of pH will affect the rate of reaction in an enzyme catalyzed reaction. The hypothesis was correct in stating that the increased pH will increase the rate of reaction. In Test one the qualitative color was only at nine after five minutes of timing the reaction. In the next experiment the qualitative color was at ten, for some, before the four minute mark. This being the case, the experiment does not support our hypothesis. The graph above shows the rate of reaction. Within the first three minutes of the reaction drops at a very rapid rate. Then, the next two minutes the rate of reaction has a huge increase. After this wave the reaction steadily decreases until it halts. Since, at the beginning of the reaction the reaction dropped, the color changed very slowly. With the increase the color changed very dramatically. In conclusion, increasing amount pH to the reaction did help increase the time it took to finish.
Ms. Lahr
Ap-Biology
September 17, 2013
Title:
The Effect of Abiotic and Biotic Factors on Enzymatic Reactions
Problem:
Will increased pH affect the rate of reaction in an enzyme catalyzed reaction?
Hypothesis:
Increased pH substance will increase the rate of reaction
Table:
Table 1: Baseline of peroxide
Time (min)
Qualitative color
1:00
5
2:00
6
3:00
7
4:00
8
5:00
9
Table 2: effect of increased pH on peroxidase
Increased pH by 5mL
Color intensity
2
10
5
10
1
10
8
9
11
10
3
10 Time in minutes
Graph:
Graph1: The effect of pH on the enzyme Peroxidase
Y- value rate of reaction X- value = pH of solution
Conclusion: This experiment was to test if increased amount of pH will affect the rate of reaction in an enzyme catalyzed reaction. The hypothesis was correct in stating that the increased pH will increase the rate of reaction. In Test one the qualitative color was only at nine after five minutes of timing the reaction. In the next experiment the qualitative color was at ten, for some, before the four minute mark. This being the case, the experiment does not support our hypothesis. The graph above shows the rate of reaction. Within the first three minutes of the reaction drops at a very rapid rate. Then, the next two minutes the rate of reaction has a huge increase. After this wave the reaction steadily decreases until it halts. Since, at the beginning of the reaction the reaction dropped, the color changed very slowly. With the increase the color changed very dramatically. In conclusion, increasing amount pH to the reaction did help increase the time it took to finish.