Lab Report Chromatography

Topics: Color, Green, Purple Pages: 3 (558 words) Published: April 9, 2013
Lab report:
Apparatus:
* 1 chromatography sheet
* Pipette
* 30cm2 Beaker
* 3 crushing bowls
* Pencil
* Ruler
* Scissors

Materials:
* 2-3 g leafs of “Feldsalat”
* 2-3 g of leak
* 2-3 g of Brussel sprouts
* 30 ml. of acetone
* 6 g of fine sand
* 6 g sodium carbonate
* 0.25 g water

Results

Leaf type| Pigment colors|
| Distance Between starting line and pigment in mm ±0.5| | Light yellow| Yellow-green| Dark Green| Light Blue/green| Dark Blue| orange| Leek| 2.0| 7.0| 9.0| 17.0| 27.0| 46.0|
“Feldsalat”| 1.0| 4.0| 5.0| 9.0| 14.0| 14.0|
Brussel Sprouts| 1.0| 3.0| 5.0| 9.0| 24.0| 42.0|

Processed Data:
Leaf type| Pigment colors|
| Distance Between starting line and pigment in mm ±0.5| | Light yellow| Yellow-green| Dark Green| Light Blue/green| Dark Blue| Orange| Leek| 0.037735849| 0.132075472| 0.169811321| 0.320754717| 0.509433962| 0.867924528| “Feldsalat”| 0.018867925| 0.075471698| 0.094339623| 0.169811321| 0.264150943| 0.264150943| Brussel Sprouts| 0.018867925| 0.064603774| 0.094339623| 0.169811321| 0.452830189| 0.79245283|

Conclusion:
I calculated the Rf values of my experiment using the following formula: Distance to pigment/distance solvent front. Some of the colors that I observed in the chromatography paper clearly have a specific pigment name. The yellow-green color is the pigment called Chlorophyllβ. The light blue-green is the pigment called Chlorophyllα. Finally, he color orange is pigment called Caroteneα and β.

When compared to the second table, showing the Rf values of different pigments, my results are actually pretty off. The only one that is kind of close to the table values are the Brussel sprouts on the yellow-green pigment.

Evaluation:
To conduct my experiment I used several instruments of measuring that have an uncertainty value. The ruler I used had an uncertainty of 0.5 mm. Obviously it...
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