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3.01 Cell Cycle Lab Report
Safety Notes:

Always handle microscopes and glass slides carefully.
Wash your hands after handling the prepared specimens.
Materials:

Compound light microscope
Glass microscope slide with prepared onion root tip specimen Purpose:

understand and identify the stages of the cell cycle and mitosis. apply an analytical technique to estimate the relative length of each stage of the cell cycle. Hypothesis:

What do you predict you will find about the number of cells in each of the phases of the cell cycle? Provide an explanation for your prediction.
Procedure: 

Data and Observations:
Create a data table containing a tally of the number of cells observed in each of the following stages:

StageNumber of Cells in Part 1Number of Cells in Part 2
Interphase  
Prophase  
Metaphase  
Anaphase  
Telophase  
Cytokinesis  
Record any observations about the cells you observed (what does the cell look like for each stage):

Data Analysis: 
Calculate the percentage of the cell cycle spent in each stage. Number of cells in given stage ÷ total number of cells counted × 100 = % of the cell cycle spent in this stage

Create a graph that represents the time spent in each stage of the cell cycle.

Conclusion:
Be sure to answer the following reflection questions in the conclusion of your lab report:

Based on your data, what can you infer about the length of time spent in each stage of the cell cycle? What stages were the longest and shortest? Give a brief explanation of why these stages may have that time period. What is a distinguishing visible feature of each stage of the cell cycle? What differences can you see when you compare the nucleus of a dividing cell with that of a nondividing cell? If your observation had not been restricted to the tip of the onion root, how would the results be different? [ Close this window ]
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