Measuring the rate of the reaction by using different substrate structures Introduction:
Enzymes are 3-D protein biological molecules that are responsible/essential for chemical reactions. Most of these reactions require a catalyst, which increases the rate of a chemical reaction without being consumed or changed during the process. If a change occurs in a chemical reaction between the reactants and products, it is usually caused by reducing the activation energy, which increases the chemical reaction. Enzyme functions depend mostly on the environment in which the reaction is occurring. Most enzymes are highly specific. This means that most of the time a single enzyme molecule will catalyze only one chemical reaction. For an enzymatic reaction to occur, an enzyme binds to a substrate causing an enzyme-substrate complex. Catechol is a naturally occurring compound found in many vegetables and fruits. When different substrates (Catechol, Resorcinol, and Hydroquinone) are added to a solution, the rate of the enzymatic reaction will gradually change. Catechol oxidase reacts with the substrates Catechol, Resorcinol, and Hydroquinone to form a Benzoquinone, which is the product. A simplified version of the reaction can be written:
The substrates in the experiment are the reacting molecules. The only difference between the Catechol, Resorcinol and Hydroquinone is that they differ in the relative positions of the hydroxyl group. When the reaction takes place, the color of benzoquinone will be yellowish-brown. The more a product is colored, the more light it will absorb at specific wavelengths: Beer’s Law. It was hypothesized that when different substrates are added to a solution, the rate of the enzymatic reaction will change. If this is true, then as time increases, the rate of the reaction of enzymatic activity will gradually change due to the direct relationship between absorbance and concentration. The purpose of this experiment was to determine the change of...
Please join StudyMode to read the full document