Tissue Culture & Hybridoma Technology
Dr. Lawrence Flowers
27 November 2012
Receptor tyrosine kinases (RTKs) are known to be key regulators of normal cellular processes such as differentiation, migration, proliferation and survival, but they also have a critical role in the development and progression of many types of human cancers. Before the published work concerning ROR1 was available it was unknown whether other cancers besides leukemia expressed ROR1 or whether its expression had functional and clinical significance. This paper investigated whether ROR1 had any consequence to breast cancer. Using a high-affinity mAb specific for ROR1 (4A5) to examine human breast cancers, this study demonstrated that ROR1 is expressed in human breast cancers and is indicative of potential targets for breast cancer therapies. Building on prior research the authors affirmed that the ROR1 associations with human breast cancer contributed to tumor-cell growth and survival via the activation of PI3K, AKT, and cAMP-response-element-binding protein (CREB). In lieu of reading this article for review, it is apparent that certain deficiencies are observed in context composition. In the initial remarks authors do not define any relevant terms that are used throughout the paper. There is essentially not enough background information given to orient the research. Referencing prior research did however, serve as slight insight. The references given are noted in the references section of paper to guide further reading into the subject matter. To dually note, the majority of the references are recent, being published from a year range of 1992 to 2011.This information is important because the most recent data also employs established precedents. These references are used mainly in support of the research, and not necessarily any information that could be used for negation. Authors did...