Jell-O Petri Dish Lab

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  • Topic: Laboratory glassware, Microbiology equipment, Petri dish
  • Pages : 6 (2365 words )
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  • Published : February 25, 2013
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Jell-O Lab

Guiding Question: Are some laundry detergents better than others?

Purpose: We intend to test if the cost of a detergent affects its quality but putting 3 drops of Jell-O into 25mL of Jell-O and examining how much of the Jell-O dissolves.

Hypothesis: If the cost of the detergent increases, then the amount of Jell-O dissolved will increase by 0.3 cm.

Materials/Equipments:
* 4-1mL Pipettes
* 5cm long standard drinking straw with a diameter of .6cm. * Jell-O brand gelatin in lime
* Insulated pad
* 5-10mL of distilled water
* A clear, flat, metric ruler
* 1 pair of standard cutting scissors
* 1 blank printer paper
* 1 Crucible Tongs
* 1 medium point black or blue sharpie
* 2 1L Beaker
* Hot plate
* Stirring Rod
* 4 Petri dishes -8.9cm in diameter
* 1 Standard refrigerator
* 1 Celsius thermometer
* 250mL graduated cylinder
* 25mL graduated cylinder
* 10 Paper towels
* Flat surface of at least 40cm by 40cm
* Sink
* #2 Pencil with eraser
* Tooth pick of 6.5cm in length
* 91% Isopropyl alcohol
* Card board box
* 4 Test tubes
* 1 Test tube rack
* 1 Test tube brush
* 3 sheets of lined paper for data

Procedures:
1. Gather your Jell-O, distilled water, 1 L beaker, hot plate, stirring rod, Petri dishes, thermometer, 250mL graduated cylinder, and 25 mL graduated cylinder. 2. Measure 471 mL of distilled water into the 1 L beaker, set beaker on hot plate, and turn hot plate to #10 heat setting. 3. Heat water until boiling. Water should be above 100 degrees C; this takes about 10 minutes. 4. Remove beaker from heat and place on an insulated pad, add 2 jello packets, and mix with stirring rod. 5. Measure 471 mL of cool distilled water using a 250 mL graduated cylinder. Add the cool water to the boiling jello solution. 6. Then, measure 25 mL of the jello solution into the bottom of each Petri dish. Cover immediately to prevent evaporation. 7. Carefully stack Petri dishes and refrigerate overnight. 8. In the morning, take out your test tube rack, 4 test tubes, Gain detergent, Ecos detergent, Tide detergent, and your 4 pipettes. 9. Place the test tubes into the test tube rack at a 90 degree angle, in separate wells, with the open sides of the tubes facing upwards. 10. Take your Gain detergent and pour it into the first test tube so that it fills up the tube 3cm, then place it into the first well from the left in the test tube rack. 11. Take your Ecos detergent and pour it into the open side of the second test tube so that it fills up the tube 3cm, then place the tube into the second well from the left, of the test tube rack, with the open side still facing upward. 12. Take your Tide detergent and pour it into, the open side of the third test tube so that it fills up the tube 3cm, then place the tube into the third well from the left, of the test tube rack, with the open side still facing upward. 13. Now place three of the pipettes into each of the three test tubes with the smaller, thinner part of the tube in the detergents. 14. Now take your clean 1L beaker and fill it half way with distilled water. Place the last pipette into the water with the small, thin part of the pipette in the water. 15. Place these things to the side, out of the way; they will be used later on. 16. Remove your petri dishes from the fridge; begin careful to hold the petri dishes containing jello on the sides of the dish to prevent melting the jello. Then place 1 petri dish on a blank sheet of printer paper and set the others on the side, not stacked and apart from each other. 17. Trace the petri dish onto the paper and slide the dish off of the paper onto the side with the other dishes. 18. Using your clear ruler and pencil, draw 2 lines on your tracing that divide the tracing into 4 even quadrants. Keeping in mind that the diameter is 8.9cm. 19. Put a circle or dot...
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