Its a fun paper
Lab 12: RFLP Analysis Using Gel Electrophoresis
Rahul Truter Jordan Higley
Purpose Statement: To see the uses of Gel electrophoresis and how the RFLP analysis can be used in legal justice.
Principles:
1. Define the following terms:
a. An enzyme that cuts DNA at certain base patterns
b. A process that separates fragments of DNA by length and charge
c. The fragments of DNA after a Restriction Enzyme is used on a strand of DNA
2. Ecor1 5' GAATTC 3
Bam HI GATCC
3. They are separated on the size of the molecule and the charge of the molecule.
4. Melting it allows for the gel to be manipulated into any shape and cooling it will leave it in a semi-rigid shape.
5. It creates a unique profile to a person that only can be seen when their DNA is spread apart on gel electrophoresis. Also with a DNA fingerprint it has unique base sequences that are not found in any other person.
6. Which suspect committed the crime? How do you know?
Conclusion Questions:
1. Restriction enzymes are the bacteria's form of an 'immune system' against viruses (which can infect bacteria. When viruses try to insert their own DNA into a bacteria's genome, the restriction enzymes detect this foreign DNA and cut it out so that the viruses can't replicate and kill the cell.
2. If everything in this lab stayed the same except that you placed your gel in the electrophoresis chamber with the wells containing the DNA next to the red electrode instead of the black, what would happen? Explain. The DNA would flow backwards instead of forward from its well, meaning that the DNA would basically pile onto one-another since the wells are at the very end of the gel. The negative DNA would flow towards the red electrode, which is positive.
3. If you have a restriction enzyme that cuts a piece of DNA at two recognition sites, how many fragments would you see on a gel? Explain. You would have 3 since the enzyme would cut away one end of the DNA, then the other end of the DNA, then all you
Rahul Truter Jordan Higley
Purpose Statement: To see the uses of Gel electrophoresis and how the RFLP analysis can be used in legal justice.
Principles:
1. Define the following terms:
a. An enzyme that cuts DNA at certain base patterns
b. A process that separates fragments of DNA by length and charge
c. The fragments of DNA after a Restriction Enzyme is used on a strand of DNA
2. Ecor1 5' GAATTC 3
Bam HI GATCC
3. They are separated on the size of the molecule and the charge of the molecule.
4. Melting it allows for the gel to be manipulated into any shape and cooling it will leave it in a semi-rigid shape.
5. It creates a unique profile to a person that only can be seen when their DNA is spread apart on gel electrophoresis. Also with a DNA fingerprint it has unique base sequences that are not found in any other person.
6. Which suspect committed the crime? How do you know?
Conclusion Questions:
1. Restriction enzymes are the bacteria's form of an 'immune system' against viruses (which can infect bacteria. When viruses try to insert their own DNA into a bacteria's genome, the restriction enzymes detect this foreign DNA and cut it out so that the viruses can't replicate and kill the cell.
2. If everything in this lab stayed the same except that you placed your gel in the electrophoresis chamber with the wells containing the DNA next to the red electrode instead of the black, what would happen? Explain. The DNA would flow backwards instead of forward from its well, meaning that the DNA would basically pile onto one-another since the wells are at the very end of the gel. The negative DNA would flow towards the red electrode, which is positive.
3. If you have a restriction enzyme that cuts a piece of DNA at two recognition sites, how many fragments would you see on a gel? Explain. You would have 3 since the enzyme would cut away one end of the DNA, then the other end of the DNA, then all you