Organic Chemistry Laboratory II, 10 February 2011
Introduction and Objectives
The purpose of this lab was to obtain a pure sample of clove oil from ground cloves using steam distillation, perform infrared spectroscopy on the sample, and analyze the IR to identify the major constituent in the clove oil. The first day the clove oil was removed from the ground cloves into an aqueous solution then removed from the water using DCM. The sample was left in a sealed container for one week and then isolated by drying the sample and removing the DCM. After the clove oil was isolated it was analyzed using IR spectroscopy. Experimental
Isolation of Clove Oil: The steam distillation apparatus was assembled and then the water in the distilling column was turned on. 5.000 g of ground clove was obtained and added to the 500 mL round bottom flask. 50.0 mL of distilled water was obtained and added to the same 500 mL round bottom flask. 100.0 mL of distilled water was obtained and added to the separatory funnel. The Bunsen burner was attached to the gas line and the gas was turned on. The Bunsen burner was lit and then used to “paint” the bottom of the 500 mL round bottom flask with water and ground cloves in it. The solution was heated to a medium boil. The vapors began to travel up into the distilling head and then began to accumulate in the distilling column. The distillate was collected in a 250 mL flask. The solution was heated until around 100 mL of distillate was collected in the receiving flask and the distillate was mostly clear and odorless. The gas and Bunsen burner were turned off. The steam distillation apparatus was allowed to cool and then disassembled. The distillate was poured into a clean, dry separatory funnel. 20.0 mL of DCM was added to the separatory funnel. The separatory funnel was capped and inverted allowing the solution to mix and the gas was released from the top. The bottom layer of the...