Isolation of the Major Component of Clove Oil
Experiment 7- Isolation of the Major Component of Clove Oil
Pre-Lab Questions: 1.) Briefly explain the concept of steam distillation. What is the difference between a simple distillation and a steam distillation? When a mixture of two immiscible liquids are distilled it is referred to as codistillation. This process is referred to as steam distillation when one of the liquids is water. This distillation is used to separate organic liquids from natural products and reaction mixtures in which the final product results in high boiling residues such as tars, inorganic salts, and other relatively involatile components. It is useful in isolating volatile oils from various parts of plants and not useful in the final purification of a liquid because it cannot separate components that have similar boiling points. The difference between simple distillation and steam distillation is that more water may be added during the distillation during steam distillation. Simple distillation allows a separation between two compounds of 60-70 degrees C or greater, or can be used when separating a liquid from non-volatile solids. Steam distillation is used to distill organic compounds that would decompose during simple distillation. 2.) The major component of clove oil is a compound call Eugenol. One of the minor components is called Acetyleugenol. Draw the structures of both of these compounds (Wikipedia may be helpful). What would be a good estimate for the pKa of Eugenol? Eugenol, pKa=10.19 at 25 degrees C.
3.) The recovered clove oil will be analyzed by Infrared Spectroscopy. What are the basic principles of Infrared Spectroscopy (can be included in the introduction if you prefer). How will we be able to distinguish between Eugenol and Acetyleugenol by IR sprectroscopy? Molecule atoms act like they are connected by flexible springs instead of rigid bonds which resemble a ball and stick model. The components of the molecule can
Pre-Lab Questions: 1.) Briefly explain the concept of steam distillation. What is the difference between a simple distillation and a steam distillation? When a mixture of two immiscible liquids are distilled it is referred to as codistillation. This process is referred to as steam distillation when one of the liquids is water. This distillation is used to separate organic liquids from natural products and reaction mixtures in which the final product results in high boiling residues such as tars, inorganic salts, and other relatively involatile components. It is useful in isolating volatile oils from various parts of plants and not useful in the final purification of a liquid because it cannot separate components that have similar boiling points. The difference between simple distillation and steam distillation is that more water may be added during the distillation during steam distillation. Simple distillation allows a separation between two compounds of 60-70 degrees C or greater, or can be used when separating a liquid from non-volatile solids. Steam distillation is used to distill organic compounds that would decompose during simple distillation. 2.) The major component of clove oil is a compound call Eugenol. One of the minor components is called Acetyleugenol. Draw the structures of both of these compounds (Wikipedia may be helpful). What would be a good estimate for the pKa of Eugenol? Eugenol, pKa=10.19 at 25 degrees C.
3.) The recovered clove oil will be analyzed by Infrared Spectroscopy. What are the basic principles of Infrared Spectroscopy (can be included in the introduction if you prefer). How will we be able to distinguish between Eugenol and Acetyleugenol by IR sprectroscopy? Molecule atoms act like they are connected by flexible springs instead of rigid bonds which resemble a ball and stick model. The components of the molecule can