Isolation and Identification of Extended Spectrum Beta Lactamse Producers from Various Clinical Samples

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Identification and Isolation of ESBL producers from Various Samples MSc. (Microbiology)
Resistant organisms – an emerging threat to the community and hospitals. Klebsiella pneumoniae is a leading cause of infection in patients with underlying diseases. Cephalosporin resistant Klebsiella spp. are called as extended spectrum beta lactamase (ESBL) producers. ESBL enzyme are plasmid mediated, capable of hydrolyzing a wide variety of β lactams, including third generation cephalosporins and aztreonams. Aims and objectives

Isolation and identification of K.pneumoniae from various medical samples. Detection of ESBL producers by carrying out AST on the isolated organisms using antibiotic discs of cephalothin, cefuroxime, Cefoperazone, imipenem, ceftazidime/clavulanate and aztreonam. Determination of genetic pattern of the isolates using pipracillin-tazobactum, ampicillin-sulbactum and ceftazidime/clavulanate. Materials and methods

Samples used for isolation Urine sample
Identification of ESBL producers Antibiotic Susceptibility Testing is carried out. Organisms: Methods
Sample collection. Processing of sample according to the guidelines provided Antibiotic Susceptibility Testing on the isolated samples is carried out Extended spectrum beta lactamase test was carried out. Results

Pink muciod colonies obtained on MacConkey’s Agar plate. Isolated organisms were tested to identify the organism by carrying out Biochemical tests from the standard charts of Berges Mannual. Results obtained were

In urine, of 50, 38 samples showed presence of infection. In blood no sample showed any infection.
in sputum of 25, 19 samples showed the presence of infection. From 30 Pus samples, 25 showed presence of infection
AST for detection of ESBL production on the isolated samples was carried out....
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