Investigatory Project in Biology

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ANTIMICROBIAL ACTIVITY OF CHILI (CAPSICUM ANNUM) EXTRACT

An Investigatory Research

Presented to

Mrs.

Presented by

CHAPTER I INTRODUCTION

Background of the Study

. Capsicum annuum is a domesticated species of the plant genus Capsicum native to southern North America and northern South America. Chili has been a part of the human diet in the Americas since at least 7500 BC. There is archaeological evidence at sites located in southwestern Ecuador that chili peppers were domesticated more than 6000 years ago and is one of the first cultivated crops in the Central and South Americas that is self-pollinating. The plant is a perennial, but usually grown as an annual, with a densely branched stem. The plant reaches 0.5–1.5 m (20–60 in). The species is a source of popular sweet peppers and hot chili fruit, and numerous varieties are cultivated around the world. Despite being a single species, Capsicum annuum has many cultivars, with a variety of names. In American English it is commonly known as a chili pepper or bell pepper. Hot peppers are used in medicine as well as food in Africa. It is employed in medicine, in combination with Cinchona in intermittent and lethargic affections, and also in atonic gout, dyspepsia accompanied by flatulence, tympanitis, paralysis etc. Its most valuable application appears however to be in cynanche maligna and scarlatina maligna, used either as a gargle or administered internally. In this research, antimicrobial activity of chili will be studied. Certain bacteria and fungal species will be the focus of the experiment. Bacteria such as Staphylococcus aureus and fungi like Trichophyton rubrum

Statement of the Problem

The main problem of this study is to determine the antimicrobial activity of chili (Capsicum annum) extract. This study also intends to answer the following.
1. The effectiveness of the chili extract as an antimicrobial. 2. The response of the bacteria species to the chili extract. 3. The response of the fungal species to the chili extract.

Significance of the Study

This study would add knowledge in the field of science. This would also be a help in further studies about plants as antimicrobial. Science students and science teachers can use this as a source for future research with regards to this study. The public would directly benefited by this study. Infections of bacteria and fungi to humans such as pimples, athlete’s foot, skin infection and diarrhea will be aided naturally. Since chili is cheap and highly available, people can easily get and make antimicrobial extract from chili. Moreover, this study may as well be use by future researchers as a resource material. Scope and Limitation of the Study

This research would make use extract from chili as antimicrobial. Other natural extracts from other plants are excluded. Antimicrobial activity includes bacteria and fungal species. Bacteria species consist of Staphylococcus aureus while fungal species consist of Trichophyton rubrum.

Dealing with microorganisms might be exposed for contamination. Aseptic technique should be observed. Over 2 plates of microorganisms will be prepared and a many laboratory works will be done. This might lessen the effectiveness of the research data and result due to human cause. That’s why human patience and strength should be employed to avoid incorrect result.

CHAPTER II
REVIEW OF RELATED LITERATURE

Capsicum Annuum as Plant
Capsicum annuum is a domesticated species of the plant genus Capsicum native to southern North America and northern South America. The three species C. annuum, C. frutescens and C. chinense all evolved from a single common ancestor located somewhere in the northwest Brazil -...
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