Investigating an Enzyme Catalyzed Reaction
Running head: LAB 5: INVESTIGATING AN ENZYME-CATALYZED REACTION
Lab 5: Investigating an Enzyme-Catalyzed Reaction
September 24, 2014
Principles of Biology 120.601
Mrs. Annemarie Duncan
Abstract: (Burmania)
This experiment was performed in order to examine ways in which a potato catalase enzyme reacts to various assays with differing variables. To do so a baseline assay (undiluted extract and room temperature H2O2) was used within the experiment with only one other variable changed in the other assays. These variables included a boiled, frozen and then thawed, and frozen potato extract and dH2O instead of the potato extract. It was noted that the temperature and or way the potato extract was prepared effects how the enzyme with the potato will react. Therefore the results of each assay varied, suggesting there is more than one way that a catalyzed reaction can occur.
Introduction: (Burmania)
The main purpose for this experiment was to explore how an enzyme catalase caused a reaction to catalyze through doing various assays using potato extracts. Enzymes are catalysts that are crucial in helping to speed up reactions, and catalase is a common enzyme found in almost every living organism that is exposed to oxygen. For this experiment, potatoes were used because they were previously known to have significant catalase activity. The potato catalase was mixed with hydrogen peroxide because the catalase helps to breakdown the hydrogen peroxide which helps determine how long a paper disk takes to float to the surface, which in return provides the time of the complete reaction. Since various assays were mostly used in combination with the potato extract, the extract should aid in speeding up the reactions with the assays that it is used. With the following information, the various ways that the assays were prepared within the realm of temperature differences, it was hypothesized that there would be a significant rate of reaction. If the reactions
Lab 5: Investigating an Enzyme-Catalyzed Reaction
September 24, 2014
Principles of Biology 120.601
Mrs. Annemarie Duncan
Abstract: (Burmania)
This experiment was performed in order to examine ways in which a potato catalase enzyme reacts to various assays with differing variables. To do so a baseline assay (undiluted extract and room temperature H2O2) was used within the experiment with only one other variable changed in the other assays. These variables included a boiled, frozen and then thawed, and frozen potato extract and dH2O instead of the potato extract. It was noted that the temperature and or way the potato extract was prepared effects how the enzyme with the potato will react. Therefore the results of each assay varied, suggesting there is more than one way that a catalyzed reaction can occur.
Introduction: (Burmania)
The main purpose for this experiment was to explore how an enzyme catalase caused a reaction to catalyze through doing various assays using potato extracts. Enzymes are catalysts that are crucial in helping to speed up reactions, and catalase is a common enzyme found in almost every living organism that is exposed to oxygen. For this experiment, potatoes were used because they were previously known to have significant catalase activity. The potato catalase was mixed with hydrogen peroxide because the catalase helps to breakdown the hydrogen peroxide which helps determine how long a paper disk takes to float to the surface, which in return provides the time of the complete reaction. Since various assays were mostly used in combination with the potato extract, the extract should aid in speeding up the reactions with the assays that it is used. With the following information, the various ways that the assays were prepared within the realm of temperature differences, it was hypothesized that there would be a significant rate of reaction. If the reactions