At the completion of the prac, the practical experience of operating a scanning electron microscope is sufficient to operate the particular machine in the future. During the experiment, two different gold plated samples are analysed under the SEM and compositional and topographic information is identified and analysed. Both the information is derived by changing the working distance, accelerating voltage, aperture size, probe current, resolution and magnification.
To analyse samples and obtain micrographs of the surface, using a scanning electron microscope. The experimental images are compared against the different material structures and defects between the various samples at different magnifications and resolutions.
A schematic of a scanning electron microscope is shown below. The instrument can be separated into three major sections including electron-optical column', vacuum system and electronics and display system.
Figure 1: Layout off scanning Electron Microscope.
In the optical part of instrument, the first thing we notice is the need for a source of illumination. No visible photons of light are produced within the SEM but an invisible beam of electrons. The beam is produced from an electron gun' and a cross-section through a simplified electron gun is shown below.
Figure 2: Cross-section through an electron gun
A V-shaped tungsten filament (I) is heated electrically to about 2700Kand the high temperature causes many of the electrons in the tungsten to become sufficiently excited for them to escape. This process is called "thermionic emission".
Once freed the electrons would be quickly recaptured by the filament, because in losing them, it will have become positively charged. Applying a high negative voltage (typically 2-25 kV) between the filament and a nearby earthed anode disc, accelerates the electrons away from the filament and their velocity depends on the accelerating voltage and is only a fraction of the speed of light. Because of the high voltages applied to the gun, good electrical insulation (3) is required.
Enclosing the filament in a metal cylinder (2), usually called the Wenhelt cylinder or cathode, shapes the beam electrostatically, so that it emerges 10 50 ìm in diameter. Unfortunately in air, or any other atmosphere, the electrons would be scattered by collision with gas molecules and they could travel only a few millimetres. A vacuum system is connected (figure 1, 14) to the column, so as to remove most of the gas molecules from the beam's path.
The ultimate performance of the SEM is mainly limited by the diameter of the beam. In order to improve performance we must be able to control it. In figure 1 the upper two lenses, the condensers (3), control the beam's diameter and demagnification occurs, when the diameter is reduced from 50 ìm to around 5 nm. Electron lenses are very different from their optical counterparts.
Figure 3: Simplified electron lens (cross-section)
A coil of wire, with its axis aligned along the beam's path, is partially enclosed in a cylindrical iron case. There is a small gap in the inner bore of the case. When a direct current is passed through the coil an electromagnet is produced, with magnetic poles (N and S), created at the gap in the iron case. This is the polepiece gap' and it is really the magnetic lines of force, bridging this space that forms the lens.
Due to their charge, electrons are deflected as they intersect the lines of force. All electrons, entering the bore at the top of the lens, converge at a focal point (f), below the lens. A single point of focus is only produced if all the electrons have the same energy. This means the gun's accelerating voltage must be kept very stable, any variation will result in electrons of different wavelength or chromatic aberration. Changing the current through the coil changes the magnetic field strength. This in turn...