Full Length Research Paper
Characterization of industrial waste and identification of potential micro-organism degrading tributyl phosphate Trupti D. Chaudhari3, Susan Eapen2 and M. H. Fulekar1*
Department of Life Sciences, University of Mumbai, Santacruz (E), Mumbai-400 098, India. Nuclear Agriculture Biotechnology Division, Bhabha Atomic Research centre, Trombay-400 085 Mumbai, India. 3 Research Scholar, Environmental Biotechnology Laboratory, Department of Life Sciences, University of Mumbai, Santacruz (E), Mumbai-400 098, India. 2 1
Accepted 6 May, 2009
The present research study has been carried out in the waste disposal site for characterization of physical, chemical and biological parameters to assess the microbial consortium present in the contaminated site and to isolate the potential micro-organism for biodegradation of Tributyl Phosphate. The ambient conditions present in the contaminated site shows the values: pH (6.61), Temperature (35.6), Moisture (50.72%), Nutrients; Nitrogen (0.41%), Phosphorus (27.87 mg/l), and Sulphur (993.5 mg/l) respectively. The biological parameters studied indicate Dissolved Oxygen (4.58 mg/l), Biological Oxygen Demand (4.62 mg/l), Chemical Oxygen Demand (146.1mg/l).The microbial consortium identified was found to survive and multiply in the present environment conditions. Microbial consortium was sequenced and compared using BLAST, ClustalW and PHYLIP. In order to identify potential microorganism, microbial consortium was exposed to increasing concentration of Tributyl Phosphate viz. 10, 25, 50, 75 and 100 mg/l in MSM, the potential microorganism was found to survive at higher concentration and utilized it as a sole source of carbon. This organism was identified as Pseudomonas pseudoalcaligenes strain DSM 50018T using 16S rRNA sequencing. This organism was found to have high potential for degradation of Tributyl Phosphate present in Low Level Nuclear Waste. Key words: Tributyl phosphate (TBP), low level nuclear waste, 16S rRNA sequencing, industrial effluent, biodegradation. INTRODUCTION Rapid industrialization and urbanization have enhanced the levels of organic and inorganic contaminants in the environment. Nuclear wastes generated through chemical processing in nuclear industry or nuclear weapons program have also enhanced the level of organic contaminants. The waste generated from nuclear industry generally contains radio nuclides, heavy metals along with myriads of toxic organics. Several physico-chemical methods to decontaminate the nuclear waste have been established and employed. However, in Low-Level Nuclear Waste, concentrations involved are low and volumes are large. Hence physical and chemical methods cannot be practiced to decon-taminate the Low-level nuclear waste. The organics as well as inorganic chemicals present in the nuclear waste find their ways in soil-water causing environmental pollution. Most of these compounds can be inactivated or degraded by microorganisms (Kumar et al., 1996).Among them, Tri butyl phosphate (TBP) has been poorly investigated because of its low toxicity in mammals. (Healy et. al., 1995 McDonald et al., 2002).Nevertheless, its wide utilization in defoamers, plasticizers, herbicides, hydraulic fluids and as a solvent for conventional nuclear fuel processing generates large amount of wastes. This compound is very stable in the natural environment and is hardly affected by natural photolysis and hydrolysis (Environment Protec-
*Corresponding author. E-mail: firstname.lastname@example.org.
J. Toxicol. Environ. Health Sci.
tion Agency 1992). Recent studies have shown that nuclear waste contaminants can have both lethal and a sublethal effect on a variety of organisms. TBP is a known carcinogen and remain in the environment for a very long period of time. In spite...