Preview

Identifying Cis-Regulatory Motif in Co-Expressed Beetle Genes

Good Essays
Open Document
Open Document
2777 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Identifying Cis-Regulatory Motif in Co-Expressed Beetle Genes
Identifying cis-regulatory motif in co-expressed Tribolium castaneum genes and inspection of immunity conferring genes in females post-mating

Amrita Pal
(Master’s Non-thesis candidate)

Demuth Lab
April 18, 2011

Acknowledgement:

I am highly obliged to be able to perform tests and learn new techniques and concepts in the lab of Prof. Jeff Demuth, University of Texas at Arlington. He also allowed me work as a Research Assistant through a semester and was an excellent teacher. I express my gratitude towards the Biology Department at UTA which provided me assistantship during my stay. I sincerely want to thank Prof. Esther Betran, Andre Pires de Silva for serving my committee and being helpful teachers. Besides, I want to acknowledge the contribution of faculty especially Prof. Trey Fondon, Cedric Feschotte, Shawn Christensen, James Robinson, Ellen Pritham, postdocs Anna Williford, Miguel Gallach and students Prince Eldon, Marcel Guibot, Amrita Naidu, Eric Watson, Emma Mujica, Heath Blackmon, Heather Hawkins, Susana Domingues, Mahima Varma, Blaine Thompson, Jainy Tharun who through their examples gave me inspiration and guided me through their knowledge so that I could carry on graduate studies. My thanks to Prof Laura Mydlarz for allowing me conduct an experiment in her lab. I want to thank the Graduate School that provided me travel stipends for presenting at an International Conference.

Also I am indebted to my parents for everything they did and are still doing to support me. I am grateful for all the criticism and feedback I received from fellow people here at UT Arlington, this helped me become a better individual.

Abstract

Computational experimentation was done to find cis-regulatory motifs in co-expressed Tribolium casteneum Nimblegen microarray data (available from earlier work done in the same lab). The different categories of data available were male and female gonads, pupae and adults.

You May Also Find These Documents Helpful

  • Good Essays

    That can be shown by the differences in the multiple sequence arrangement as shown above. As the same arrangement shows there would be a difference in the function of the male and female. That should not affect the main function, however, that should not be the case. Looking at the annotated gene above, it can be determined that they are similar, but also different. The L. cuprina and L. sericata have a different number of introns and exons. They also all contain a different number of total base pairs. That along with the non-existent UTR in the female gene. As discussed the gene functions in the acetylation of histone K4K16. That is the only function that can be determined. That is based off of the phenylogenic tree and the data found on NCBI and all of the other databases sited below. The gene was found to function in Chromatin binding in Xenopus tropicalis. Where as in Mus musculus the function is Chromatin binding, chromatin modification, histone-H4-K16 acetylation. This is very similar to the function in Rattus norvegicus which is histone H4-K16 acetylation. Complex neurobehavioral disorder…

    • 941 Words
    • 4 Pages
    Good Essays
  • Powerful Essays

    This experiment was carried out to test the role of gene sequences Srb-16 and F34B1.3 in the chemosensation of C. elegans. Using RNAi treatment, the genes were inhibited from encoding their specific proteins and the resulting effect was analyzed to determine whether or not the genes play a role in chemotaxis of diacetyl. Diacetyl (DA) is a toxic buttery smelling odorant normally attractive to C. elegans. By observing the worms post RNAi treatment in their ability to detect and move towards DA, conclusions were drawn as to whether the gene sequence…

    • 3094 Words
    • 13 Pages
    Powerful Essays
  • Powerful Essays

    Stowe, K. A., L. L. Hester, and Vieyra, M. L. 2008. Biology 101 Lab Manual. Hayden- McNeil Publishing Inc., Plymouth, Michigan, USA.…

    • 2415 Words
    • 10 Pages
    Powerful Essays
  • Better Essays

    Keith, E., Messing, C,. Schmitt, E., Feingold, J. (2010). Laboratory Exercises in Biology (3rd ed.). Dubuque, IA: Kendall Hunt Publishing Company.…

    • 2179 Words
    • 9 Pages
    Better Essays
  • Good Essays

    Developers of Lab: Adapted by James Kirby JD726, Jennifer Mortellaro JD449, and James Prockup JD575 from a publication by the Department of Biological Sciences at Western Michigan University, Kalamazoo, MI…

    • 1444 Words
    • 6 Pages
    Good Essays
  • Good Essays

    This may be due to the fact that Pc3 encodes a more severe antimorphic protein that makes the animal more susceptible to relevant genetic interactors. Identification of a genetic interaction between Myb and Pc is consistent with Myb’s role in stabilizing H3K27me3, the histone mark associated with facultative heterochromatin which is the mark that is bound by Pc. We also observed a complete lack of GFP negative larvae in the Ash1 crosses, indicating a particularly strong genetic interaction between Myb and either of two Ash1 alleles. This may be due to Myb’s vital role in activation of transcription for hundreds of target…

    • 652 Words
    • 3 Pages
    Good Essays
  • Powerful Essays

    Diffusion and Osmosis

    • 3022 Words
    • 13 Pages

    * Morgan, G; Carter, M; Dickey, J. 2010. Investigating Biology, Pearson, United States of America.…

    • 3022 Words
    • 13 Pages
    Powerful Essays
  • Powerful Essays

    Peroxisome in Animal Cell

    • 1969 Words
    • 8 Pages

    References: [3] Neil A. Campbell, Jane B. Reece, Lisa A Urry, Michael L.Cain, Steven A. Wasserman, Peter V. Minorsky et al. Biology. Eighth edition. Pearson international edition. 2008. P.110-111.…

    • 1969 Words
    • 8 Pages
    Powerful Essays
  • Powerful Essays

    DROSOPHILA CULTURE We will study basic principles of Mendelian inheritance with the use of the fruit fly, Drosophila melanogaster [the name means “black-bodied fruit-lover”]. Drosophila was one of the first organisms to be studied genetically: its small size, short life cycle (10 ~14 days at 25oC), high reproductive rate (an adult female can lay 400-500 eggs in 10 days), and ease of culture and genetic manipulation have made it perhaps the best understood animal genetic system. Many different species, and a large number and wide variety of naturally-occurring and artificially-induced genetic variants are available. The partial genetic map in Appendix B describes the location of all the mutations used in crosses and lab questions. VIRGIN FEMALES All female flies used in controlled genetic crosses must be “virgins”. Female flies are capable of mating as early as 8 hours after emerging from the pupae stage and are polyandrous, that is, capable of mating with several males. Once mated, females can retain viable sperm for several days and this will confuse the results of a subsequent controlled mating. To prevent this, all adult flies are removed from the culture bottle about 7 hours prior to lab time, so that all newly hatched flies will remain virgin. BASIC GENETICS The karyotype of Drosophila comprises four pairs of chromosomes, of which three pairs are autosomes and one pair are sex chromosomes. Female Drosophila are XX, and males XY. A gene is a heritable factor that controls the expression of some trait, which may be morphological, behavioural, molecular, etc. Each such gene occupies a specific physical locus (pl. loci) on a particular chromosome. Variant forms of these loci are termed alleles. Gene, locus, and allele are often used more or less interchangeably, and this can lead to confusion. Gene is the popular and most general term, and is most appropriate when the inherited basis of a trait is emphasized, e.g., a “gene” for…

    • 3748 Words
    • 15 Pages
    Powerful Essays
  • Good Essays

    Cholesterol

    • 682 Words
    • 3 Pages

    Reece, J.B., Urry, L.A., Cain, M.L., Wasserman, S.A., Minorsky, P.V. & Jackson, R.B. (2011). Campbell Biology. (9th ed.). San Francisco: Pearson Education Inc.…

    • 682 Words
    • 3 Pages
    Good Essays
  • Better Essays

    Molecular Genetics

    • 9782 Words
    • 40 Pages

    Sci. Rev., 12 (5) May 2000, pp. 22-25. An account of gene function, including a comparison of gene regulation in pro- and eukaryotes.…

    • 9782 Words
    • 40 Pages
    Better Essays
  • Better Essays

    2) Campbell, N. A., J. B. Reece, and L. G. Mitchell.(2012) Biology. 9th edition. Pearson Benjamin/Cummings, Menlo Park, CA, USA. Pg. 151-155.…

    • 1263 Words
    • 6 Pages
    Better Essays
  • Good Essays

    Eukaryotic Genomes

    • 1157 Words
    • 5 Pages

    Gene regulation in eukaryotes is a complicated process compared to prokaryotes. For example, 85% of the DNA is transcribed or translated in prokaryotes, while only 20% in eukaryotes. This reveals that transcription alone does not account for gene expression due to the complexity of eukaryotes.…

    • 1157 Words
    • 5 Pages
    Good Essays
  • Powerful Essays

    Diabetes

    • 1577 Words
    • 5 Pages

    Campbell, N. A., Reece, J. B., Urry, L. A., Cain, M. L.,Wasserman, S. A., Minorsky, P. V. and Jackson, R. B. (2008) Biology. 8 ed, San Francisco: Pearson Benjamin Cummings, pp.983-984…

    • 1577 Words
    • 5 Pages
    Powerful Essays
  • Powerful Essays

    water cycle

    • 1415 Words
    • 5 Pages

    I would like to thank my family for all of their support also I would like to thank all of my teachers for all of the encouragement and most of all I would like to thank god for keeping me calm with all the stress I had.…

    • 1415 Words
    • 5 Pages
    Powerful Essays